Abstract：Objective: To investigate the role and underlying mechanism of T-cadherin in multidrug resistance of melanoma. Methods: The sensitivity of the cisplatin-resistant B16F10 (CDDP-R B16F10) melanoma cell line to dacarbazine (DTIC) and paclitaxel (PTX) was assessed. The effects of T-cadherin in combination with DTIC and PTX on cell proliferation and migration were evaluated using the MTT assay and wound healing assay. qRT-PCR and Western blot analyses were performed to determine the impact of T-cadherin on the expression of multidrug resistance gene 1 (MDR1) and Survivin in CDDP-R B16F10 cells. Results: The resistance index (RI) of CDDP-R B16F10 cells was 3.46 for DTIC and 1.46 for PTX. Compared to other groups, the combination of T-cadherin with DTIC and PTX significantly reduced the OD value and wound healing rate (P<0.05). The relative mRNA and protein expression levels of MDR1 and Survivin were significantly higher in CDDP-R B16F10 cells than in parental B16F10 cells (P<0.05). However, T-cadherin transfection markedly decreased MDR1 and Survivin expression at both the mRNA and protein levels in CDDP-R B16F10 cells compared to the non-transfected groups (P<0.05). Conclusion: The multidrug resistance of CDDP-R B16F10 cells is induced by prolonged exposure to escalating doses of CDDP. T-cadherin enhances the sensitivity of CDDP-R B16F10 cells to DTIC and PTX and reverses multidrug resistance by downregulating MDR1 and Survivin expression.
陆海涛, 李雪飞, 牛艳东, 吴景良, 赵运华. T-钙黏蛋白在黑色素瘤多药耐药中的作用机制研究[J]. 河北医学, 2025, 31(6): 929-934.
LU Haitao, LI Xuefei, NIU Yandong, et al. Mechanistic Study of T-Cadherin in Multidrug Resistance of Melanoma. HeBei Med, 2025, 31(6): 929-934.
[1] 梁后杰,黄海辉.细胞黏附介导的肿瘤多细胞耐药[J].中华医学杂志,2004,84(24):2142-2143. [2] 陆海涛,李雪飞,刘利君,等.T-钙黏蛋白联合顺铂对黑色素瘤顺铂耐药株的作用研究[J].天津医药,2019,47(5):459-463. [3] 陆海涛,孙立新,陆洁,等.T-钙黏蛋白对黑素瘤侵袭力的影响[J].中国皮肤性病学杂志,2011,25(5):329-332. [4] 刘巍峰,杨发军,牛晓辉.黑色素瘤前哨淋巴结活检和区域清扫的研究进展[J].中华肿瘤杂志,2019,41(7):481-485. [5] 储文梅,熊旭东,何淼,等.白藜芦醇对人胃癌耐药细胞SGC-7901/5-FU生物学效应的影响及其作用机制[J].中国癌症防治杂志,2021,13(3):231-236. [6] Wright S,Burkholtz SR,Zelinsky C,et al.Survivin expression in luminal breast cancer and adjacent normal tissue for immuno-oncology applications[J].Int Mol Sci,2023,24(14):11827. [7] Mahmoud A M,Zekri W,Khorshed E N,et al.Prognostic significance of survivin expression in pediatric ewing sarcoma[J].Pediatr Hematol Oncol,2022,39(1):16-27. [8] Mandic D,Nezic L,Amdzic L,et al.Overexpression of MRP1/ABCC1,Survivin and BCRP/ABCC2 predicts the resistance of diffuse large B-cell lymphoma to R-CHOP treatment[J].Cancers (Basel),2023,15(16):4106. [9] Han T,Sha H,Ji J,et al.Depletion of Survivin suppresses docetaxel-induced apoptosis in HeLa cells by facilitating mitotic slippage[J].Sci Rep,2021,11(1):2283. [10] Chen XQ,Yang S,Li ZY,et al.Effects and mechanism of downregulation of survivin expression by RNA interference on proliferation and apoptosis of lung cancer cells[J].Mol Med Rep,2012,5(4):917-922.
2025, Vol. 31 
冀公网安备13080202000786号