妊娠期糖尿病孕妇外周血miR-372-3p miR-27a水平与胰岛素抵抗及新生儿结局的相关性研究

doi:10.3969/j.issn.1006-6233.2025.06.18

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摘要 目的: 观察妊娠期糖尿病(gestational diabetes mellitus,GDM)患者外周血短链非编码RNA(microRNA,miRNA)-372-3p、miR-27a水平变化与孕妇胰岛素抵抗(insulin resistance,IR)及新生儿结局的关联性。方法: 纳入2022年6月至2024年5月本院收治的102例GDM患者,同期选取102正常孕妇进行对照,测定入组者外周血miR-372-3p、miR-27a水平,并根据新生儿结局情况分为正常结局组和不良结局组,收集患者一般资料及检测信息,绘制ROC曲线分析miR-372-3p、miR-27a及胰岛素抵抗水平对GDM孕妇新生儿不良结局的预测价值。结果: GDM组外周血miR-372-3p、miR-27a及IR指数高于对照组,差异有统计学意义(P<0.05);Pearson相关性分析显示:GDM患者miR-372-3p、miR-27a表达水平与IR指数呈正相关,相关性有统计学意义(r=0.518、0.616,P<0.05);线性回归分析显示:年龄、体质量指数及miR-372-3p、miR-27a表达水平是GDM患者IR指数的影响因素,关联性有统计学意义(P<0.05)。新生儿不良结局组GDM孕妇miR-372-3p、miR-27a表达水平及IR指数高于正常结局组GDM孕妇,差异有统计学意义(P<0.05);ROC曲线显示:miR-372-3p、miR-27a表达水平及IR预测GDM孕妇新生儿结局的AUC分别为0.740、0.806、0.669。结论: GDM外周血miR-372-3p、miR-27a表达升高促进了患者IR发生风险,同时对评估新生儿结局有一定的应用价值。

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	马倩倩
	马丹
	陈东颖
	牛三强

关键词 ：妊娠期糖尿病, 短链非编码RNA, 胰岛素抵抗, 新生儿结局

Abstract：Objective: To observe the association of changes of peripheral blood short-chain non-coding RNA (miRNA)-372-3p and miR-27a with insulin resistance (IR) and neonatal outcomes in patients with gestational diabetes mellitus (GDM). Methods: 102 patients with GDM in the hospital were included from June 2022 to April 2024, and 102 normal pregnant women were selected for control. The levels of miR-372-3p and miR-27a in peripheral blood of the enrolled patients were measured, and the patients were divided into normal outcome group and adverse outcome group according to the neonatal outcomes. The general data and detection information of patients were collected, and ROC curve was drawn to analyze the predictive value of miR-372-3p, miR-27a and insulin resistance on neonatal adverse outcomes of pregnant women with GDM. Results: The peripheral blood miR-372-3p, miR-27a and IR index in GDM group were significantly higher than those in normal pregnant women group the difference was statistically significant (P<0.05). Pearson correlation analysis indicated that the expression levels of miR-372-3p and miR-27a in GDM patients were positively correlated with IR index, and the correlations were statistically significant (r=0.518, 0.606, P<0.05). Linear regression analysis demonstrated that age, body mass index and expression levels of miR-372-3p and miR-27a were influencing factors for IR index in GDM pregnant women, and the associations were statistically significant (P<0.05). The expression levels of miR-372-3p and miR-27a and IR index of GDM pregnant women in neonatal adverse outcome group were higher than those in normal outcome group, and there were statistical differences (P<0.05). ROC curve showed that the AUCs of miR-372-3p, miR-27a and IR in predicting neonatal outcomes of GDM pregnant women were 0.740, 0.806 and 0.669 respectively. Conclusion: The increased expressions of miR-372-3p and miR-27a in peripheral blood of GDM patients promote the risk of IR, and have certain application value on evaluating neonatal outcomes.

Key words： Gestational diabetes mellitus Short-chain non-coding RNA Insulin resistance Neonatal outcomes

基金资助:安徽省卫生健康科研项目,(编号:AHWJ2023BAa20129);2022年度亳州市卫生健康科研立项项目,(编号:bzwj2022b009)

引用本文:

马倩倩, 马丹, 陈东颖, 牛三强. 妊娠期糖尿病孕妇外周血miR-372-3p miR-27a水平与胰岛素抵抗及新生儿结局的相关性研究[J]. 河北医学, 2025, 31(6): 973-978.
MA Qianqian, MA Dan, CHEN Dongying, et al. Correlation of Peripheral Blood miR-372-3p and miR-27a Levels with Insulin Resistance and Neonatal Outcome in Pregnant Women with Gestational Diabetes Mellitus. HeBei Med, 2025, 31(6): 973-978.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.06.18 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I6/973

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