Abstract：Objective: To investigate the expression of microRNA-214-3p (miR-214-3p) and thrombospondin 2 (THBS2) in cervical cancer tissues and their relationship with pathological parameters and prognosis. Methods: A total of 84 cervical cancer patients who underwent total hysterectomy or radical hysterectomy in our hospital from January 2019 to March 2021 were enrolled. The expression levels of miR-214-3p and THBS2 mRNA in cervical cancer and adjacent tissues were detected by real-time quantitative PCR. THBS2 protein expression was assessed by immunohistochemistry. The binding sites between miR-214-3p and THBS2 were predicted using the TargetScan database, and Pearson correlation analysis was performed to determine the correlation between miR-214-3p and THBS2 expression in cervical cancer tissues. Based on the median expression levels, cervical cancer tissues were divided into high/low miR-214-3p and THBS2 mRNA expression groups. Kaplan-Meier survival curves were plotted to evaluate progression-free survival (PFS) in cervical cancer patients with high/low miR-214-3p and THBS2 mRNA expression. Multivariate Cox regression analysis was performed to identify factors influencing patient prognosis, using the prognosis of cervical cancer patients as the dependent variable. Results: The expression level of miR-214-3p was significantly lower in cervical cancer tissues (0.99±0.13) than in adjacent tissues (1.55±0.18), while THBS2 mRNA expression was higher in cancer tissues (1.13±0.07) compared to adjacent tissues (0.78±0.12) (t=-22.522 and 23.443, all P<0.001). A binding site was identified at positions 3934-3941 in the 3′ untranslated region of THBS2 for miR-214-3p. A negative correlation was observed between miR-214-3p and THBS2 expression in cervical cancer tissues (r=-0.830, P<0.001). The expression of miR-214-3p and THBS2 mRNA was significantly associated with tumor differentiation, FIGO stage, and lymph node metastasis (all P<0.05). During a 3-year follow-up, the progression-free survival rate was 65.48% (55/84). Patients with high miR-214-3p expression had a higher 3-year PFS rate, while those with high THBS2 mRNA expression had a lower 3-year PFS rate (Log-rank χ2=14.120 and 10.350, all P<0.05). Independent risk factors for death in cervical cancer patients included poor differentiation (HR=4.532, 95% CI: 1.230~16.707), FIGO stage III (HR=6.038, 95% CI: 1.712~21.290), lymph node metastasis (HR=9.004, 95% CI: 2.167~37.422), and THBS2 mRNA ≥1.11 (HR=6.521, 95% CI: 1.474~28.841). High miR-214-3p expression (≥1.02) was an independent protective factor (H=0.141, 95% CI: 0.035~0.568) (all P<0.05). Conclusion: Low expression of miR-214-3p and high expression of THBS2 mRNA in cervical cancer tissues are associated with unfavorable pathological characteristics and poor prognosis.
闫琼琼, 李亚萍, 朱海军, 王晓娟. 宫颈癌组织中miR-214-3p THBS2的表达及临床意义[J]. 河北医学, 2025, 31(7): 1152-1158.
YAN Qiongqiong, LI Yaping, ZHU Haijun, et al. Expression and Clinical Significance of miR-214-3p and THBS2 in Cervical Cancer Tissues. HeBei Med, 2025, 31(7): 1152-1158.
2025, Vol. 31 
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