Abstract：Objective: To investigate the expression changes of microRNA-491-5p (miR-491-5p) and zinc finger protein 703 (ZNF703) in breast cancer tissues and their relationship with prognosis. Methods: A total of 153 breast cancer patients (breast cancer group) who underwent surgical resection in our hospital from January 2017 to August 2019, and 153 patients with benign breast tumors who underwent benign breast lesion resection during the same period (control group), were included. Real-time quantitative polymerase chain reaction was used to detect the expression of miR-491-5p and ZNF703 mRNA in breast cancer and benign lesion tissues. Online databases were used to predict the binding sites between miR-491-5p and ZNF703, and the correlation between miR-491-5p and ZNF703 mRNA expression in breast cancer tissues was analyzed using Pearson correlation analysis. Based on the average expression levels of miR-491-5p and ZNF703 mRNA in breast cancer tissue, patients were divided into high-expression and low-expression groups. Kaplan-Meier survival curves were plotted for breast cancer patients with different miR-491-5p and ZNF703 expression levels, and multivariate Cox regression analysis was performed to examine the relationship between miR-491-5p and ZNF703 mRNA expression and patient prognosis. Results: Compared with the control group, miR-491-5p was under-expressed, while ZNF703 mRNA was over-expressed in the breast cancer group (P<0.05). A binding site was identified at the 3'-untranslated region (127-133) of ZNF703 for miR-491-5p, and the two showed a negative correlation in breast cancer tissues (P<0.05). Breast cancer tissues with a tumor diameter ≥2 cm, low differentiation, TNM stage Ⅲ-Ⅳ, lymph node metastasis, or distant metastasis showed lower miR-491-5p expression and higher ZNF703 mRNA expression compared to those with a tumor diameter <2 cm, medium/high differentiation, TNM stage Ⅰ-Ⅱ, no lymph node metastasis, or no distant metastasis (P<0.05). The 5-year overall survival rate of 153 breast cancer patients was 77.12% (118/153). Patients with high miR-491-5p expression had a higher 5-year survival rate than those with low miR-491-5p expression, while those with high ZNF703 mRNA expression had a lower survival rate compared to patients with low ZNF703 mRNA expression (P<0.05). Multivariate Cox regression analysis showed miR-491-5p ≥0.86 was identified as an independent protective factor, while ZNF703 mRNA ≥1.46 was an independent risk factor for breast cancer patient mortality (P<0.05). Conclusion: miR-491-5p is under-expressed and ZNF703 mRNA is over-expressed in breast cancer tissues, which are associated with poor pathological features and prognosis. They may serve as novel biomarkers for evaluating breast cancer prognosis.
周爱萍, 杨帆, 吴兰弟, 林柳妹. 乳腺癌组织中miR-491-5p ZNF703的表达变化及与预后关系[J]. 河北医学, 2025, 31(9): 1514-1520.
ZHOU Aiping, YANG Fan, WU Landi, et al. Expression Changes of miR-491-5p and ZNF703 in Breast Cancer Tissues and The Relationship with Prognosis. HeBei Med, 2025, 31(9): 1514-1520.
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2025, Vol. 31 
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