Abstract：Objective: To explore the effect of microRNA-32-5p (miR-32-5p) on the malignant biological behavior of glioma cells by targeting the regulation of E2F transcription factor 7 (E2F7). Methods: This study analyzed 45 paired glioma and adjacent tissues from surgically treated patients (from March 2023 to August 2024) using qRT-PCR to measure miR-32-5p and E2F7 mRNA expression. In vitro, U87MG cells were divided into seven groups: NC, miR-NC, miR-32-5p mimic, sh-NC, sh-E2F7, miR-32-5p mimic+pcDNA-NC, and miR-32-5p mimic+pcDNA-E2F7. Functional assays included clonogenic, flow cytometry (apoptosis), wound healing, and Transwell (migration/invasion) tests. qRT-PCR was used to assess miR-32-5p/E2F7 expression, while Western blot was used to analyze E2F7, Cyclin D1, PCNA, Caspase-3, Bax, MMP-2, and TIMP-1 protein levels. The miR-32-5p/E2F7 targeting relationship was validated by dual-luciferase reporter assay. Results: Compared with adjacent tissues, the miR-32-5p in cancer tissues was clearly reduced, while the E2F7 mRNA was clearly increased (P<0.05). For the NC group, there were no clear changes in various indicators in the miR-NC group and sh-NC group (P>0.05). For the miR-NC group or sh-NC group, the miR-32-5p mimic group or sh-E2F7 group showed a decrease in the proliferation, migration, and invasion abilities of U87MG cells. The E2F7 mRNA, E2F7, Cyclin D1, PCNA, and MMP-2 proteins were clearly reduced, while the apoptosis rate, miR-32-5p, and the Caspase-3, Bax, and TIMP-1 proteins were clearly increased (P<0.05). For the miR-32-5p mimic+pcDNA-NC group, the miR-32-5p mimic+pcDNA-E2F7 group showed increased proliferation, migration, and invasion abilities of U87MG cells. The E2F7 mRNA, E2F7, Cyclin D1, PCNA, and MMP-2 proteins were increased, while the apoptosis rate, miR-32-5p, and the Caspase-3, Bax, and TIMP-1 proteins were reduced (P<0.05). The dual luciferase assay demonstrated a targeted relationship between miR-32-5p and E2F7 (P<0.05). Conclusion: MiR-32-5p can inhibit the malignant biological behavior of glioma cells by targeting and downregulating E2F7.
孙兴旺, 梁云, 王冠朋, 胡博, 汤海群, 梁良. miR-32-5p靶向调控E2F7对脑胶质瘤细胞恶性生物学行为的影响[J]. 河北医学, 2025, 31(10): 1650-1657.
SUN Xingwang, LIANG Yun, WANG Guanpeng, et al. Effect of miR-32-5p on Malignant Biological Behavior of Glioma Cells by Targeting Regulation of E2F7. HeBei Med, 2025, 31(10): 1650-1657.
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2025, Vol. 31 
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