淫羊藿苷对大鼠骨髓间充质干细胞体外成骨分化及PI3K/AKT通路的影响

doi:10.3969/j.issn.1006-6233.2025.12.015

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摘要 目的: 探讨淫羊藿苷(ICA)对大鼠骨髓间充质干细胞(BMSCs)体外成骨分化及磷脂酰肌醇3激酶(PI3K)/丝氨酸-苏氨酸蛋白激酶(AKT)通路的影响。方法: 将第3代大鼠BMSCs随机分为Control组(DMEM培养基中培养)、地塞米松(DXMS)组(在含有10-6moL/L DXMS的DMEM培养基中培养)、L-ICA、M-ICA、H-ICA组(DXMS诱导的DMEM培养基+10-8、10-7、10-6moL/L的ICA)、ICA+740Y-P组(DXMS诱导的DMEM培养基+10-6moL/L的ICA+30μmoL/L PI3K激活剂740Y-P)。CCK8法检测大鼠BMSCs增殖;茜素红染色检测骨向诱导(在终浓度为100nM DXMS+50μM维生素+2mM L-谷氨酰胺+10mM β-甘油磷酸酯的DMEM培养基中培养)的大鼠BMSCs矿化能力;ELISA试剂盒检测大鼠BMSCs中ALP、ROS、SOD的表达;Western blot检测大鼠BMSCs中PI3K/AKT通路蛋白表达。结果: DXMS组胞浆内染色较浅,A450值、ALP、SOD低于Control组,ROS、p-PI3K/PI3K、p-AKT/AKT高于Control组(P<0.05);L-ICA组、M-ICA组、H-ICA组胞浆内加深,A450值、ALP、SOD高于DXMS组,ROS、p-PI3K/PI3K、p-AKT/AKT低于DXMS组(P<0.05);ICA+740Y-P组胞浆内染色变浅,A450值、ALP、SOD低于H-ICA组,ROS、p-PI3K/PI3K、p-AKT/AKT高于H-ICA组(P<0.05)。结论: ICA可能抑制PI3K/AKT通路,促进大鼠BMSCs的成骨分化。

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	季伟
	刘远航
	秦陈林
	欧宜林

关键词 ：骨髓间充质干细胞, 淫羊藿苷, 成骨分化, 磷脂酰肌醇3激酶, 丝氨酸-苏氨酸蛋白激酶

Abstract：Objective: To investigate the effects of icariin (ICA) on in vitro osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) and the phosphatidylinositol 3-kinase (PI3K)/serine threonine protein kinase (AKT) pathway. Methods: The third generation of rat BMSCs were randomly assigned into the control group (cultured in DMEM medium), dexamethasone (DXMS) group (cultured in DMEM medium supplemented with 10-6mol/L DXMS), L-ICA, M-ICA, H-ICA groups (DXMS induced DMEM medium+10-8, 10-7, 10-6mol/L ICA), and ICA+740Y-P group (DXMS induced DMEM medium+10-6mol/L ICA+30μmol/L PI3K activator 740Y-P). CCK8 method was used to examine the proliferation of rat BMSCs. Alizarin red staining was used to examine the mineralization ability of rat BMSCs induced by bone orientation (cultured in DMEM medium with a final concentration of 100nM DXMS+50μM vitamin+2 mM L-glutamine+10mM β-glycerophosphate). ELISA kit was used to examine the expression of ALP, ROS, and SOD in rat BMSCs. Western blot was used to examine the expression of PI3K/AKT pathway proteins in rat BMSCs. Results: The cytoplasmic staining in the DXMS group was relatively light, with lower A450 values, ALP, and SOD compared to the control group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were higher than those in the control group (P<0.05); in the L-ICA group, M-ICA group, and H-ICA group, the cytoplasmic staining deepened, with higher A450 values, ALP, and SOD compared to the DXMS group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were lower than those in the DXMS group (P<0.05); in the ICA + 740Y-P group, the cytoplasmic staining became lighter, with lower A450 values, ALP, and SOD compared to the H-ICA group, while ROS, p-PI3K/PI3K, and p-AKT/AKT were higher than those in the H-ICA group (P<0.05). Conclusion: ICA may inhibit the PI3K/AKT pathway and promote osteogenic differentiation of rat BMSCs.

Key words： Bone marrow mesenchymal stem cells Icariin Osteogenic differentiation Phosphatidylinositol 3-kinase Serine threonine protein kinase

基金资助:湖北省武汉市卫健委中医药科研项目,(编号:WZ22Q14)

通讯作者: 欧宜林

引用本文:

季伟, 刘远航, 秦陈林, 欧宜林. 淫羊藿苷对大鼠骨髓间充质干细胞体外成骨分化及PI3K/AKT通路的影响[J]. 河北医学, 2025, 31(12): 2032-2037.
JI Wei, LIU Yuanhang, CAI Chenlin, et al. Effects of Icariin on in Vitro Osteogenic Differentiation and PI3K/AKT Pathway of Rat Bone Marrow Mesenchymal Stem Cells. HeBei Med, 2025, 31(12): 2032-2037.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.12.015 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I12/2032

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