An Investigation into the Effects of Hedyotis diffusa on Rats with Liver Cirrhosis Based on the Theory of Qi and Blood Differentiation and Its Influence on NF-κB Expression and Fibrosis
LIN Yan, SHI Guoqin, et al
Lanzhou Second People's Hospital, Gansu Lanzhou 730000, China
Abstract：Objective: To investigate the effects of Hedyotis diffusa on hepatic fibrosis and NF-κB expression in rats with liver cirrhosis based on the theory of Qi and Blood Differentiation. Methods: The SD rats were randomly divided into a control group, a model group, a positive drug Yishanfu group, and Hedyotis diffusa low- and high-dose groups, with 7 rats in each group. Intraperitoneal injection of carbon tetrachloride was conducted to construct a rat model of liver cirrhosis. HE staining was used to detect pathological changes in liver tissue. ELISA assay was used to detect the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL), IL-6, and TNF-α in serum. Masson staining was used to detect the degree of fibrosis in liver tissue. RT-qPCR was used to detect the mRNA expressions of α-SMA, Col1a1, and TGF-β1 in liver tissues. An immunohistochemical staining assay was used to detect the expression and localization of p65 NF-κB protein in liver tissue. Results: Compared with the control group, rats in the model group exhibited marked pathological changes indicative of liver cirrhosis in their hepatic tissue. Serum levels of ALT, AST, TBIL, IL-6, and TNF-α were significantly elevated (P<0.05). Collagen fibre proliferation in the liver tissue was markedly increased, alongside elevated expression of α-SMA, Col1a1, TGF-β1, and p65 NF-κB in the hepatic tissue (P<0.05). Compared with the model group, pathological damage in liver tissue was improved in rats from the positive drug group and the low- and high-dose Hedyotis diffusa groups. Serum levels of ALT, AST, TBIL, IL-6, and TNF-α decreased (P<0.05), collagen fibre proliferation in liver tissue diminished, and liver tissue expression of α-SMA, Col1a1, TGF-β1, and p65 NF-κB in liver tissue decreased (P<0.05). Conclusion: Hedyotis diffusa reduces fibrosis in the liver tissue of rats with cirrhosis by inhibiting inflammation, and the mechanism may be related to the inhibition of NF-κB signaling.
林燕, 史国琴, 孙园园. 基于“气血辨证”理论探讨白花蛇舌草对肝硬化大鼠的作用及对NF-κB表达纤维化的影响[J]. 河北医学, 2026, 32(1): 28-33.
LIN Yan, SHI Guoqin, et al. An Investigation into the Effects of Hedyotis diffusa on Rats with Liver Cirrhosis Based on the Theory of Qi and Blood Differentiation and Its Influence on NF-κB Expression and Fibrosis. HeBei Med, 2026, 32(1): 28-33.
2026, Vol. 32 
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