Abstract：Objective: To investigate the correlation of m5C RNA methylation modification in vestibular function and age-related hearing loss. Methods: A total of 24 inbred C57BL/6J mice were used, which were 5 months old (adult mouse group), 12 months old (middle-aged mouse group) and 20 months old (elderly mouse group), 8 mice in each group. The ROS levels in cochlea and vestibular tissues were determined by DCFH-DA (dichlorodihydrofluorescein-acetoacetate) method. Cochlea and vestibular tissues’ mitochondrial DNA integrity was determined by TAMRA probes. Cochlea and vestibular tissues’ m5C levels of GRM7 mRNA were analyzed by m5C RNA methylation immunocoprecipitation. The levels of NSUN2 in cochlea and vestibular tissues were determined by Western blot. Results: Compared with adult mice group and middle-aged mice group, the average ABR thresholds in aged mice group were increased at 8, 16 and 32 kHz (P<0.05). The relative levels of ROS in cochlear tissues and vestibular tissues were increased (P<0.05). The proportion of mtDNA containing CD (%) was decreased (P<0.05). The relative expression levels of NSUN2 were decreased (P<0.05). The relative expression levels of GRM7 Input mRNA were decreased (P<0.05). The relative expression levels of GRM7 m5C mRNA was decreased (P<0.05). In aged mice group, compared with the cochlear tissue group, the relative levels of ROS in vestibular tissue group was lower (P<0.05), and the relative expression levels of GRM7 Input mRNA and GRM7 m5C mRNA were higher (P<0.05). Conclusion: Down-regulation of m5C RNA methylation and decreased expression of NSUN2 were associated with vestibular function and age-related hearing loss.
周旭, 司峰志. m5C RNA甲基化修饰与前庭功能异常和老年性耳聋的相关性研究[J]. 河北医学, 2026, 32(1): 117-122.
Zhou Xu, Si Fengzhi. Study on the Correlation between m5C RNA Methylation Modification and Vestibular Dysfunction and Age-Related Hearing Loss. HeBei Med, 2026, 32(1): 117-122.
2026, Vol. 32 
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