Pathogenetic Characteristics of Paediatric Infectious Mononucleosis Based on Pathogen-Targeted Sequencing Technology and Correlation Analysis with EB Virus Viral Load
ZHENG Zheng, et al
Tongling First People's Hospital, Anhui Tongling 244000, China
Abstract：Objective: To detect pathogens in children with infectious mononucleosis (IM) based on pathogen-targeted sequencing (tNGS) technology, and to analyze their pathogenic characteristics and the correlation with EBV viral load. Methods: The observation group consisted of 46 children with IM caused by EBV who were admitted to the First People's Hospital of Tongling and Tongling Maternal and Child Health Hospital between June 2023 and December 2024. The control group consisted of 95 non-IM children who were admitted to our hospital during the same time period.. The tNGS was used to detect pathogens in the two groups of patients and to compare the pathogenetic characteristics of the two groups. Results: Using the tNGS technique, 32 pathogens, including 13 bacteria, 18 viruses, and 1 mycoplasma, were detected in the control group, while 24 pathogens, including 12 bacteria, 11 viruses, and 1 chlamydia, were detected in the observation group. Among the pathogens isolated using the tNGS technique, the top 3 bacterial detections in the control group were Haemophilus influenzae (n=40), Streptococcus pneumoniae (n=29), and Acinetobacter baumannii (n=31), while in the observation group the top 3 bacteria were Haemophilus influenzae (n=29), Streptococcus pneumoniae (n=22), and Staphylococcus aureus (n=21). EBV was detected in both groups, and the EBV viral load in the observation group was higher than that in the control group (P<0.05). According to correlation analysis results, the EBV virus load was positively connected with Staphylococcus aureus, Moraxella catarrhalis, Acinetobacter baumannii, Klebsiella pneumoniae, and Streptococcus angina.(P<0.05). Conclusion: tNGS technology facilitates a comprehensive understanding of the pathogenetic characteristics in children with IM, and these pathogenetic features correlate with EBV viral load, thereby providing a basis for clinical diagnosis and treatment.
[1] 刘梦佳,王然,谢正德.传染性单核细胞增多症的流行病学、免疫机制及临床管理[J].中国科学:生命科学,2024,54(12):2311-2320. [2] 黄博,董艳迎,宋琳岚.儿童传染性单核细胞增多症348例临床特征分析[J].临床儿科杂志,2024,42(8):678-683. [3] Shi T,Li J,Miao Y,et al.Adenosine deaminase as a marker for the severity of infectious mononucleosis secondary to EBV in children[J].BMC infectious diseases,2022,22(1):164. [4] 陶锋,李一荣,周艳梅,等.靶向宏基因组测序技术在不明原因肺部感染病原学诊断中的价值[J].中国病原生物学杂志,2024,19(11):1290-1294. [5] Cui Z,Wang J,Diao J,et al.Knowledge mapping of childhood infectious mononucleosis:a bibliometric analysis for the twenty-first century[J].Journal of health,population,and nutrition,2025,44(1):43. [6] 沈燕,陆建春,冯妍,等.儿童传染性单核细胞增多症临床表现及外周血Th1/Th2型细胞标志物与EB病毒-DNA载量的关系[J].中国感染与化疗杂志,2023,23(3):318-322. [7] 郭红仙,胡玉杰,尹凤蕊,等.传染性单核细胞增多症270例临床和实验室检查分析[J].中华实用儿科临床杂志,2022,37(19):1478-1481. [8] Ishiguro K,Ishikawa K,Matsuo T,et al.Bilateral sialadenitis as an uncommon initial manifestation of infectious mononucleosis[J].Internal medicine (Tokyo,Japan),2024,63(22):3105-3108. [9] Bucharskaya A B,Yanina I Y,Atsigeida S V,et al.Optical clearing and testing of lung tissue using inhalation aerosols:prospects for monitoring the action of viral infections[J].Biophysical reviews,2022,14(4):1005-1022.
2026, Vol. 32 
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