Abstract：Objective: To investigate whether electroacupuncture treatment can regulate the PI3K/AKT signaling pathway to improve the neurological function of neonatal rats with cerebral palsy (CP). Methods: Neonatal rats were randomly divided into the Sham group, CP group, electroacupuncture group, and electroacupuncture+Ly294002 (PI3K inhibitor) group. The neurological function of the neonatal rats was evaluated. The morphology of hippocampal tissue was observed by HE staining. The neuron apoptosis rate was detected by TUNEL assay. The level of myelin basic protein (MBP) in the hippocampal tissues was detected by immunohistochemistry. The levels of inflammatory factors and oxidative stress indices in the hippocampal tissues were detected. The expressions of BDNF, cleaved-caspase-3, NGF and proteins related to the PI3K/AKT signaling pathway were detected by Western blot. Results: Compared with the Sham group, in the CP group, there was obvious damage to the hippocampal cells of neonatal rats. The number of correct responses in the Y-maze test, the suspension score, the integrated optical density (IOD) level of proliferative MBP in the hippocampus, the activities of superoxide dismutase (SOD) and catalase (CAT), and the expression levels of BDNF, NGF, p-PI3K/PI3K, and p-AKT/AKT proteins were decreased. While the neuron apoptosis rate, the levels of tumor necrosis factor-α (TNF-α), interleukin-18 (IL-18) and interleukin-6 (IL-6), the content of malondialdehyde (MDA), and the expression level of cleaved-caspase-3 protein were increased (P<0.05 ). Compared with the CP group, in the electroacupuncture group, the morphological changes of hippocampal cells in neonatal rats were significantly improved. The number of correct responses in the Y-maze test, the suspension scores, the IOD level of proliferative MBP in the hippocampus, the activities of SOD and CAT, and the expression levels of BDNF, NGF, p-PI3K/PI3K, and p-AKT/AKT proteins were increased. Whereas the neuron apoptosis rate, the levels of TNF-α, IL-18 and IL-6, the content of MDA, and the expression level of cleaved-caspase-3 protein were decreased (P<0.05 ). Ly294002 could reduce the effect of electroacupuncture on the improvement of neurological function in neonatal rats with CP (P<0.05 ). Conclusion: Electroacupuncture treatment may activate the PI3K/AKT signaling pathway to alleviate hippocampal damage and improve neurological function in neonatal rats with CP.
侯红颖, 尹倩倩, 郭锐. 基于PI3K/AKT通路探讨电针对脑瘫幼鼠神经功能的改善作用[J]. 河北医学, 2026, 32(2): 213-219.
HOU Hongying, YIN Qianqian, GUO Rui. Improvement Effect of Electroacupuncture on Neurological Function in Neonatal Rats with Cerebral Palsy Based on the PI3K/AKT Signaling Pathway. HeBei Med, 2026, 32(2): 213-219.
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2026, Vol. 32 
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