Rho相关激酶在高糖诱导小鼠心脏内皮细胞衰老中的作用研究

doi:10.3969/j.issn.1006-6233.2018.02.001

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Abstract Objective: To investigate the role of Rho-associated kinase in the high glucose-induced murine heart endothelial cells (MHECs) senescence. Methods: MHECs senescence was induced by culturing with 22 mM glucose for 72 h. MHECs in control group were treated with normal culture media and the MHECs in High glucose (HG) group were treated with 22 mM glucose. MHECs in Y27632 group were pretreated with Rho-associated kinase inhibitor Y27632 before cultured in high glucose. In addition, mannitol was added to exclude the effect of osmotic pressure. The ratio of senescent cells was identified by senescence-associated β -galactosidase (SA-βG) staining and flow cytometry was used to analyze the cell cycle. The expression of senescence associated protein p21, p-MBS(Thr853) and MBS was explored by Western blot. The telomerase activity in endothelial cells and the level of nitric oxide (NO) in culture medium assay kit were detected with respective kits. Results: Compared to the control group, treatment of high glucose could significantly increase the ratio of SA-βG positive cells (44.00%±3.63% vs. 4.17%±0.70%,P＜0.01), enhance ROCK activity, increase the ratio of cells in G0/G1 stage and the expression of p21, inhibit telomerase activity of the cells and NO level in culture medium (all P<0.05). However, treatment of ROCK inhibitor Y27632 could greatly inhibit ROCK activity and effectively decrease the ratio of senescent cells (both P<0.05). Also, treatment of Y27632 could decrease the ratio of cells in G0/G1 stage and the expression of p21 and reverse the decrease of telomerase activity and NO level in cells cultured in high glucose medium (all P<0.05). Conclusion: Rho related kinase activation is involved in high glucose induced endothelial cell senescence, and inhibition of Rho related kinase can reduce the senescence of endothelial cells induced by high glucose, and provide a new direction for the prevention and treatment of diabetic angiopathy.

Key words： Rho-associated kinase Cellular senescence High glucose Endothelial cell

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	XU Changwu
	QIU Liqiang
	JIANG Hong

Cite this article:

XU Changwu,QIU Liqiang,JIANG Hong. Effects of Rho-Associated Kinase on High Glucose-Induced Murine Heart Endothelial Cell Senescence[J]. HeBei Med, 2018, 24(2): 177-181.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2018.02.001 OR http://www.hbyxzzs.cn/EN/Y2018/V24/I2/177

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