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| Expression of LncRNA MEG3 in Peripheral Blood Mononuclear Cells and Sputum of Children with Viral Bronchiolitis and Its Significance |
| ZHU Lianhai, HE Changsheng, et al |
| Jiuting Hospital in Songjiang District of Shanghai, Shanghai Songjiang District 201600, China |
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Abstract Objective: To detect the expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children with viral bronchiolitis and analyze its clinical significance. Methods: 103 children with viral bronchiolitis admitted to Jiuting hospital and Songjiang Central Hospital from February 2017 to January 2019 were selected, and 103 healthy infants and children in the same period of physical examination were selected as the control group. After collecting peripheral blood mononuclear cells and sputum, the expression of LncRNA MEG3 was detected by qRT-PCR, and its relationship with clinicopathological characteristics and prognosis was analyzed. Results: The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children in control, mild, moderate and severe groups decreased significantly in turn, and the differences between any two groups were statistically significant (P<0.05). The expression level of vascular endothelial growth factor (VEGF) mRNA in peripheral blood mononuclear cells and sputum of children in control, mild, moderate and severe groups increased significantly in turn, and the differences between any two groups were statistically significant (all P<0.05). The expression level of LncRNA MEG3 in peripheral blood mononuclear cells and sputum was negatively correlated with the expression level of VEGF mRNA (all P<0.05). The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum of children with allergic history and post-discharge asthma was significantly lower than that of children without allergic history and concurrent asthma (P<0.05). Allergic history, high expression of VEGF and low expression of LncRNA MEG3 were risk factors for adverse prognosis in children with viral bronchiolitis (all P<0.05). Conclusions: The expression of LncRNA MEG3 in peripheral blood mononuclear cells and sputum decreased significantly in control, mild, moderate and severe groups, which may be related to the occurrence and development of viral bronchiolitis and the prognosis of children.
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2020, Vol. 26 
