依托咪酯对PI3K/AKT通路及非小细胞肺癌A549细胞凋亡的影响

doi:10.3969/j.issn.1006-6233.2020.07.003

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Abstract Objective: To investigate the effect of etomidate on PI3K/AKT pathway and apoptosis of non-small cell lung cancer A549 cell. Methods: A549 cell group, 5-fluorouracil group, etomidate low-dose group and high-dose group were set up. 5-fluorouracil group, etomidate low-dose group and high-dose group were added 5-fluorouracil group and etomidate at different concentrations, and the final concentration was 100.0ug/mL, 100.0ug/mL and 200.0ug/mL for 72 hours. Cell proliferation level was assessed by cell counting kit-8, apoptotic level was measured by flow cytometry, cell invasiveness was measured by Transwell chamber, p-PI3K, p-AKT mRNA and protein levels were measured by RT-PCR and WEST-BLOT. Results: Compared with A549 cell group, the OD value, survival rate, number of perforation, p-PI3K, p-AKT gene and protein were significantly decreased in 5-fluorouracil group, low-dose and high-dose etomidate group(P<0.05), apoptotic rate were significantly increased(P<0.05). And with the increase of etomidate dosage, the OD value, survival rate, number of perforation, p-PI3K, p-AKT gene and protein were gradually decreased (P<0.05), apoptotic rate were gradually increased (P<0.05). Compared with 5-fluorouracil group, the OD value, survival rate, number of perforations, p-PI3K, p-AKT mRNA and protein in low-dose etomidate group were significantly increased(P<0.05), and apoptotic rate were significantly decreased (P<0.05); while the OD value, survival rate, number of perforation, p-PI3K, p-AKT mRNA and protein in high-dose etomidate group were significantly decreased(P<0.05), and apoptotic rate were significantly increased(P<0.05). Conclusion: Etomidate can inhibit the proliferation and invasion of lung cancer A549 cells and promote the apoptosis of lung cancer A549 cells. Its mechanism is related to the inhibition of PI3K/AKT pathway activation and transmission by reducing the levels of p-PI3K, p-AKT mRNA and protein phosphorylation by etomidate.

Key words： Etomidate PI3K/AKT pathway Non-small cell lung cancer Apoptosis

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2020.07.003 OR http://www.hbyxzzs.cn/EN/Y2020/V26/I7/1066

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