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| Detection and Correlation Analysis of Gal-3 IL-33 Expression, MP Load and Neutrophil Ratio in Alveolar Lavage Fluid of Children with MPP |
| FAN Mengjie, YANG Yao |
| Yichang First People's Hospital / People's Hospital of Three Gorges University, Hubei Yichang 443000, China |
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Abstract Objective: To investigate the levels of galectin-3 (Gal-3), interleukin-33 (IL-33), Mycoplasma pneumoniae (MP) -DNA, neutrophil proportion and clinical significance in alveolar lavage fluid of children with mycoplasma pneumoniae pneumonia (MPP). Methods: A total of 102 children with MPP who were treated in our hospital from January 2020 to March 2022 were selected as the observation group, including 59 children with mild disease and 43 children with severe disease, while 60 children with bronchial foreign bodies were selected as the control group, and the differences in Gal-3, IL-33, MP-DNA load, neutrophils, etc. in alveolar lavage fluid in each group were compared, the changes of Gal-3, IL-33, MP-DNA load and neutrophil in alveolar lavage fluid in the observation group before and after treatment were analyzed. Results: Gal-3, IL-33, MP-DNA load, neutrophils and macrophages in alveolar lavage fluid of the observation group were (18.82 ± 3.10) pg/ml, (20.01 ± 3.43) pg/ml, (5.94 ± 1.01) log copy/ml, (18.21 ± 3.11)% and (88.14 ± 9.92)%, respectively, which were significantly higher than those in the control group (P<0.05). Gal-3, IL-33, MP-DNA load and neutrophil in alveolar lavage fluid of severe children in the observation group were (20.32 ± 3.02) pg/ml, (22.03 ± 3.05) pg/ml, (7.07 ± 1.70) log copy/ml and (20.31 ± 2.88)% respectively, which were significantly higher than those of mild children (P<0.05). Gal-3, IL-33, MP-DNA load, neutrophils and macrophages in alveolar lavage fluid of mild and severe children in the observation group decreased after treatment compared with those before treatment (P<0.05). Gal-3, IL-33, neutrophils in alveolar lavage fluid were positively correlated with MP-DNA load (P<0.05), while no significant correlation was found in the rest. Conclusion: Gal-3, IL-33, MP-DNA load and the proportion of neutrophils in the alveolar lavage fluid of children with MPP are significantly increased, which is related to the severity of the disease, and Gal-3, IL-33, neutrophils in the alveolar lavage fluid are positively related to MP-DNA load.
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2023, Vol. 29 
