芍药苷调节SIRT1/PGC-1α/Nrf2信号通路对过氧化氢诱导皮肤成纤维细胞氧化应激损伤的影响

doi:10.3969/j.issn.1006-6233.2024.09.03

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Abstract Objective: To investigate the effect of paeoniflorin (PF) on oxidative stress damage induced by hydrogen peroxide (H₂O₂) in skin fibroblasts (HSF) by regulating the silent information regulator 2 homologue 1 (SIRT1)/peroxisome proliferator activated receptor-gamma coactivator-1α (PGC-1α)/nuclear factor erythroid-derived 2-related factor 2 (Nrf2) signaling pathway.Methods: HSF cells were studied and separated into the Control group, H₂O₂ group, L-PF, M-PF, H-PF group, and PF+EX527 group. CCK-8 was applied to detect HSF cell proliferation. The β-galactosidase staining method was applied to detect HSF cell aging. Flow cytometry was used to detect apoptosis of HSF cells. ELISA method was used to detect the expression of ROS, SOD, GSH, and MDA in HSF cells. WB was used to detect the expressions of SIRT1, PGC-1α, and Nrf2 proteins in HSF cells.Results: The survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression of HSF cells in the H₂O₂ group were lower than in the control group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were higher than in the control group (P<0.05). Compared with the H₂O₂ group, the survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression were increased in the L-PF group, M-PF group, and H-PF group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were decreased (P<0.05). The survival rate, SOD, GSH, SIRT1, PGC-1α, and Nrf2 expression in the PF+EX527 group were lower than in the H-PF group, the proportion of aging cells, apoptosis rate, ROS, and MDA expression were higher than in the H-PF group (P<0.05).Conclusion: PF can inhibit H₂O₂-induced oxidative stress damage in HSF cells, and its mechanism may be achieved by activating the SIRT1/PGC-1α/Nrf2 signaling pathway.

Key words： Paeoniflorin Silent information regulator 2 homologue 1 Peroxisome proliferator activated receptor-gamma coactivator-1α Nuclear factor erythroid-derived 2-related factor 2 Skin fibroblasts Oxidative stress damage

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	HUANG Yan
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HUANG Yan,YANG Yanqing,et al. Effect of Paeoniflorin on Hydrogen Peroxide-Induced Oxidative Stress Damage in Skin Fibroblasts by Regulating the SIRT1/PGC-1α/Nrf2 Signaling Pathway[J]. HeBei Med, 2024, 30(9): 1428-1433.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.09.03 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I9/1428

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