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| Exogenous Nerve Growth Factor Activates the Wnt3α/β-Catenin Signaling Pathway to Promote Fracture Healing |
| ZHAO Yanzhe, LIU Jian, SHI Xiaoyun |
| Shijiazhuang Third Hospital, Hebei Shijiazhuang 050000, China |
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Abstract Objective: To analyze the effect of exogenous nerve growth factor on the activation of Wnt3α/β-catenin signaling pathway in promoting fracture healing. Methods: Select 40 SPF grade SD rats, 10 rats as the control group, and 30 rats to construct a tibial fracture model. A total of 27 rats were successfully modeled and divided into a model group of 9 rats, a low-dose group of 9 rats, and a high-dose group of 9 rats. Observe the pathological histology, bone calcium and phosphorus levels, hemorheological indicators, BMD, callus diameter, angiogenesis related factors, inflammatory factor levels, mRNA expression levels of key genes Wn3a, β -catenin, and GSK-3β in the Wnt3 α/β-catenin pathway, and expression levels of key proteins Wnt3a, β-catenin, and GSK-3β in the Wnt3 α/β-catenin pathway in each group of rats. Results: Compared with the control group, the other three groups showed a decrease in bone calcium, bone phosphorus, BMD, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and an increase in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β related mRNA expression, and GSK-3β expression. Compared with the model group, the low and high dose groups showed an increase in bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3 α, β - catenin related mRNA expression, Wnt3α, β-catenin expression, and a decrease in whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3 β related mRNA expression, and GSK-3β expression; And the high-dose group showed the highest levels of bone calcium, bone phosphorus, BMD, callus diameter, angiogenesis related factors, Wnt3α, β-catenin related mRNA expression, Wnt3α, β-catenin expression, and the lowest levels of whole blood viscosity, plasma viscosity, inflammatory factor levels, GSK-3β - related mRNA expression, and GSK-3β expression (P<0.05). Conclusion: The intervention of exogenous nerve growth factor on tibial bone can promote the healing of fracture in rats, the mechanism of which may be related to the activation of Wnt3α/β-catenin signaling pathway, and has a good effect.
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2024, Vol. 30 
