党参炔苷调节TXNIP/NLRP3信号通路对骨关节炎大鼠软骨损伤的影响

doi:10.3969/j.issn.1006-6233.2024.11.04

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Abstract Objective: To investigate the effect of Lobetyolin on cartilage injury in osteoarthritis (OA) rats by regulating the TXNIP/NLRP3 signaling pathway.Methods: The rats were randomly divided into sham group, model group, Lobetyolin low-dose group, Lobetyolin high-dose group, and Lobetyolin high-dose +HY-N2485 (NLRP3 activator) group, with 10 rats in each group. ELISA was used to measure TNF-α, IL-1β, IL-4, CTX-I, CTX-II levels in rat serum. HE and saffron O-green staining were applied to observe morphological changes in articular cartilage tissue. The Mankin score was used to assess the degree of cartilage degeneration. TUNEL was applied to observe apoptosis of rat chondrocytes. Cartilage tissue NO, MDA, and SOD levels were measured. Western blot was used to detect the protein expression of MMP-3, MMP-13, Cleaved-caspase-3, TXNIP and NLRP3 in rat articular cartilage tissue.Results: Compared with the Sham group, the cartilage tissue structure of the rats in the model group was obviously damaged, the serum IL-1β, TNF-α, CTX-I, CTX-II levels, Mankin score, apoptosis rate, NO and MDA levels in cartilage tissue, MMP-3, MMP-13, Cleaved-caspase-3, TXNIP, NLRP3 protein expression levels increased, the serum IL-4 level and cartilage tissue SOD level decreased (P<0.05). Compared with the model group, the degree of cartilage tissue structure destruction in the Lobetyolin low-dose and high-dose groups was reduced, the serum IL-1β, TNF-α, CTX-I, CTX-II levels, Mankin score, apoptosis rate, NO and MDA levels in cartilage tissue, MMP-3, MMP-13, Cleaved-caspase-3, TXNIP, NLRP3 protein expression levels decreased, the serum IL-4 level and cartilage tissue SOD level increased (P<0.05). HY-N2485 could partially reverse the ameliorating effect of Lobetyolin on cartilage injury in OA rats (P<0.05).Conclusion: Lobetyolin can reduce cartilage inflammation, oxidative stress and apoptosis, and alleviate cartilage tissue damage in OA rats, which may be achieved by inhibiting the TXNIP/NLRP3 signaling pathway.

Key words： Osteoarthritis Cartilage damage Lobetyolin TXNIP/NLRP3 signaling pathway CTX-Ⅰ CTX-Ⅱ

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.11.04 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I11/1778

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