长链非编码 RNA UCA1调控食管鳞状细胞癌细胞生长和转移的功能研究

doi:10.3969/j.issn.1006-6233.2024.12.02

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Abstract Objective: To investigate the effects of LncRNA UCA1 on the in vivo and in vitro growth and metastasis of esophageal squamous cell carcinoma cells.Methods: qRT-PCR was used to detect the expression of LncRNA UCA1 in human esophageal epithelial cells (HEEC), human esophageal squamous cell carcinoma cells (EC109, EC9706, KYSE150, and KYSE450). EC109 and EC9706 cells were divided into si-NC group and si-UCA1 group. CCK8 and flow cytometry were used to detect cell proliferation and cell cycle, Transwell method was used to detect cell migration and invasion. Western blot was used to detect the relative expression levels of E-cadherin, Vimentin, and MMP-9 in cells. 1×10⁶ EC109 cells of si-NC and si-UCA1 groups were inoculated into the left axilla of BALB/c-A nude mice, after 4 weeks of feeding, the tumor volume and weight of the mice were observed and recorded.Results: The expression of LncRNA UCA1 in EC109, EC9706, KYSE150, and KYSE450 was significantly higher than that in HEEC cells (P<0.001). Compared with the si-NC group, the proliferation, migration, and invasion abilities of EC109 and EC9706 cells in the si-UCA1 group were significantly reduced (P<0.05), cell cycle was significantly arrested (P<0.05), the expression of E-cadherin was significantly upregulated (P<0.001), and the expression of Vimentin and MMP-9 were significantly downregulated (P<0.001). The tumor volume and weight of mice in the si-UCA1 group were significantly lower than those in the si-NC group (P<0.001). Conclusion: Inhibiting LncRNA UCA1 can inhibit the proliferation, migration, and invasion ability of esophageal squamous cell carcinoma cells, as well as their growth in mice.

Key words： LncRNA UCA1 Esophageal squamous cell carcinoma Metastasis

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	TANG Chengqiong
	Baerxiaguli Zabihula
	LU Yanrong

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TANG Chengqiong,Baerxiaguli Zabihula,LU Yanrong. Study on the Regulation of Growth and Metastasis of Esophageal Squamous Cell Carcinoma Cells by LncRNA UCA1[J]. HeBei Med, 2024, 30(12): 1945-1951.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2024.12.02 OR http://www.hbyxzzs.cn/EN/Y2024/V30/I12/1945

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