LncRNA FGD5-AS1通过miR-103a-3p/RNF38轴影响结肠癌细胞的恶性生物学行为

doi:10.3969/j.issn.1006-6233.2025.01.003

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Abstract Objective: To investigate the effect of long-chain non coding RNA FGD5 antisense RNA1 (LncRNA FGD5-AS1) on the malignant biological behavior of colon cancer cells via targeting the miR-103a-3p/RNF38 axis. Methods: Fluorescence quantitative PCR was used to detect the expression of LncRNA FGD5-AS1 and miR-103a-3p in human colon epithelial cells and human colon cancer SW480. StarBase and Targeted Scan databases were applied to analyze the binding sites between LncRNA FGD5-AS1, miR-103a-3p, and RNF38, and further validation was used by dual luciferase reporter for gene detection. SW480 cells were randomly grouped into control group, pcDNA NC group, pcDNA-FGD5-AS1 group, si-NC group, si-FGD5-AS1 group, si-FGD5-AS1+inhibitor NC group, and si-FGD5-AS1+miR-103a-3p inhibitor group. The cell counting kit-8 (CCK-8) assay, flow cytometry, Transwell assay, fluorescence quantitative PCR, and Western blot were applied to detect cell proliferation, apoptosis, invasion, FGD5-AS1, miR-103a-3p levels, and RNF3 protein expression level. Results: Compared with human colonic epithelial cells, the expression level of LncRNA FGD5-AS1 in SW480 cells was significantly reduced (1.00±0.00 vs 0.48±0.10, t=12.737, P<0.05), while the expression level of miR-103a-3p was significantly increased (1.00±0.00 vs 1.61±0.18, t=8.301, P<0.05). LncRNA FGD5-AS1 could target and negatively regulate miR-103a-3p expression (P<0.05). MiR-103a-3p could target and positively regulate RNF38 expression (P<0.05). Compared with the pcDNA NC group, the level of FGD5-AS1 in SW480 cells of the pcDNA-FGD5-AS1 group significantly increased (0.98±0.10 vs 2.34±0.45, t=7.227, P<0.05), and the apoptosis rate significantly increased (8.64±1.32 vs 16.21±2.75, t=6.079, P<0.05), while the expression levels of miR-103a-3p (1.01±0.12 vs 0.42±0.07, t=10.403, P<0.05), RNF38 protein expression levels (0.59±0.09 vs 0.32±0.17, t=3.438, P=0.006), cell proliferation activity (0.63±0.09 vs 0.34±0.06, t=6.567, P<0.05), and cell invasion ability (112.63±14.94 vs 43.82±5.67, t=10.548, P<0.05) significantly decreased. The change trend of the si-FGD5-AS1 group was opposite to that of the pcDNA-FGD5-AS1 group, and miR-103a-3p inhibitor could reverse the changes of the si-FGD5-AS1 group (P<0.05). Conclusion: Interference with LncRNA FGD5-AS1 may promote RNF38 protein expression via targeting upregulate miR-103a-3p, thereby enhancing proliferation and invasion abilities of colon cancer cells, and reducing apoptosis rate. Upregulation of LncRNA FGD5-AS1 can inhibit the malignant biological behavior of colon cancer cells.

Key words： Colon cancer cells Long-chain non coding RNA FGD5 antisense RNA1 MicroRNA-103a-3p Ring finger protein 38

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.01.003 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I1/15

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