幽门螺杆菌对5种抗生素的耐药率及与相关耐药基因的关系研究

doi:10.3969/j.issn.1006-6233.2025.01.019

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Abstract Objective: To investigate the drug resistance rates of Helicobacter pylori (Hp) to five antibiotics [metronidazole (MTZ), clarithromycin (CLA), amoxicillin (AMX), levofloxacin (LEV), furazolidone (FZ)] and its correlation with related drug resistance genes in Bazhou People’ s Hospital of Korla City. Methods: One hundred and fifty-two gastric mucosal biopsy samples in Bazhou People’ s Hospital of Korla City from March 2021 to May 2024 were collected for Hp identification, isolation and culture, and the typing detection of serum antibody Hp. The drug resistance rate of Hp to five antibiotics was detected by E-test drug sensitivity test. The related genotypes of Hp resistance to five antibiotics and the correlation between each drug resistance genotype and drug resistance phenotype were analyzed with gene sequencing technology. Results: A total of 57 Hp strains were isolated from 152 gastric mucosal biopsy samples, with a positive isolation rate of 37.50% (57/152), including 50 strains (87.72%) of Hp-antibody type I and 7 strains (12.28%) of Hp-antibody type II. 6.00% of Hp strains among 50 strains of Hp-antibody type I were resistant to five antibiotics, and 74.00% of Hp strains were resistant to at least one antibiotic. The drug resistance rates of Hp to MTZ, LEV, CLA, AMX and FZ were 64.00%, 30.00%, 68.00%, 24.00% and 80.00%, respectively. The main genotype loci of Hp resistance to MTZ, LEV, CLA and AMX were rdxA (C148T+T184G), gyrA (C261G/A), 23S rRNA (T2182C) and Pbp1b (C1667G+A1684G), and the mutation rates were 58.00%, 18.00%, 64.00% and 16.00% respectively. The mutation probability of genotype locus Pord (G353A+A356G+C357T) of Hp resistance to FZ was 18.00%, and the mutation probabilities of A41G locus and A122G+C349A/G locus of gene Oord were 80.00% and 80.00% respectively. The mutations of LEV resistance gene gyrA, CLA resistance gene 23S rRNA (A2142G+A2143G) and AMX resistance gene Oord were more common in Hp-resistant strains, and the probability of occurrence was significantly higher than that of Hp-sensitive strains (P<0.05). Conclusion: Bazhou population in Korla City has a certain Hp infection rate, and the infected Hp strains have high drug resistance rates to five common antibiotics. The mutations of drug resistance genes corresponding to each antibiotic are related to the drug resistance phenotype.

Key words： Helicobacter pylori Antibiotics Resistance rate Gene mutation

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.01.019 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I1/106

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