LncRNA SNHG1调节miR-330-5p/HMGA2轴对胃癌细胞迁移侵袭和化疗敏感性的影响

doi:10.3969/j.issn.1006-6233.2025.04.01

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (2472 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To study the impacts of long non-coding RNA small nucleolar RNA host gene 1 (LncRNA SNHG1) on the migration,invasion and chemotherapy sensitivity of gastric cancer cells by regulating miR-330-5p/high mobility group A2 (HMGA2) axis. Methods: MGC-803 cells were cultured in vitro and randomly grouped into control group,si-SNHG1 group,pc-NC+miR-330-5p-NC group,and si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; cell scratch and Transwell invasion test were performed to detect cell migration and invasion in each group.MGC-803/oxaliplatin (OXA) cells were cultured in vitro and randomly grouped into control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; CCK-8,Edu staining,and TUNEL staining experiments were conducted to detect cell proliferation and apoptosis in each group.The MGC-803/OXA transplanted tumor nude mouse model was constructed and randomly grouped into a control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and processing,the tumor volume and weight were measured.Targeted validation was performed using dual luciferase reporter gene assay. Results: Compared with the control group,the expression of LncRNA SNHG1,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p increased (P<0.05).Compared with the si-SNHG1 group,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p decreased (P<0.05).Compared with the control group and OXA group,the expression of LncRNA SNHG1,the expression of HMGA2,cell viability,proliferation rate,tumor volume and weight in the OXA+si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p and apoptosis rate increased (P<0.05); there was no obvious change in all indicators of OXA group cells compared to the control group (P>0.05).Compared with the OXA+si-SNHG1 group,the expression of HMGA2,cell viability,proliferation rate,tumor volume,and weight in the OXA+si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p and apoptosis rate decreased (P<0.05).LncRNA SNHG1 was able to target the downregulation of miR-330-5p expression in MGC-803 and MGC-803/OXA cells,and miR-330-5p was able to target the downregulation of their HMGA2 expression. Conclusion: Silencing LncRNA SNHG1 can attenuate the expression of HMGA2 by up-regulating miR-330-5p,thereby inhibiting the migration and invasion of gastric cancer cells,enhancing their chemotherapy sensitivity,and enhancing the killing effect of OXA on them.

Key words： Gastric cancer LncRNA SNHG1 MiR-330-5p/HMGA2 Migration Invasion

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	HE Yiming
	JIN Ailian
	ZHOU Yipei
	et al

Cite this article:

HE Yiming,JIN Ailian,ZHOU Yipei, et al. Impacts of LncRNA SNHG1 on Migration Invasion and Chemotherapy Sensitivity of Gastric Cancer Cells by Regulating the miR-330-5p/HMGA2 Axis[J]. HeBei Med, 2025, 31(4): 529-537.

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.04.01 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I4/529

[1] Chen Y,Yin J,Zhao L,et al.Reconstruction of the gastric cancer microenvironment after neoadjuvant chemotherapy by longitudinal single-cell sequencing[J].Transl Med,2022,20(1):563-576.
[2] Makino H,Nomura S,Kogo H,et al.Role of collagen gel droplet-embedded culture-drug sensitivity testing (CD-DST) for assessing the sensitivity of gastric cancer to chemotherapy drugs combined with other cancer therapeutic drugs[J].Nippon Med Sch,2022,89(4):412-421.
[3] Xu J,Xu Y,Ye G,et al.LncRNA-SNHG1 promotes paclitaxel resistance of gastric cancer cells through modulating the miR-216b-5p-hexokianse 2 axis[J].Chemother,2023,35(6):527-538.
[4] Saproo S,Sarkar S S,Gupta E,et al.MiR-330-5p and miR-1270 target essential components of RNA polymerase I transcription and exhibit a novel tumor suppressor role in lung adenocarcinoma[J].Cancer Gene Ther,2023,30(2):288-301.
[5] Bai L,Dong K,Tong D,et al.LncRNA HIT000218960 enhances resistance to 5-fluorouracil by promoting HMGA2 and activating the AKT/mTOR/P70S6K pathway in gastric cancer cells[J].Exp Ther Med,2022,24(2):527-536.
[6] Ge P,Cao L,Zheng M,et al.LncRNA SNHG1 contributes to the cisplatin resistance and progression of NSCLC via miR-330-5p/DCLK1 axis[J].Exp Mol Pathol,2021,120(11):104633-104641.
[7] Cheng F,Wang L,Yi S,et al.Long non-coding RNA SNHG1/microRNA-195-5p/Yes-associated protein axis affects the proliferation and metastasis of gastric cancer via the Hippo signaling pathway[J].Funct Integr Genomics,2022,22(5):1043-1055.
[8] Zhang M,Yang L,Hou L,et al.LncRNA SNHG1 promotes tumor progression and cisplatin resistance through epigenetically silencing miR-381 in breast cancer[J].Bioengineered,2021,12(2):9239-9250.
[9] Zhao S,Xu F,Ji Y,et al.Circular RNA circ-CD44 regulates chemotherapy resistance by targeting the miR-330-5p/ABCC1 axis in colorectal cancer cells[J].Histol Histopathol,2023,38(2):209-221.
[10] Yang S,Zou C,Li Y,et al.Knockdown circTRIM28 enhances tamoxifen sensitivity via the miR-409-3p/HMGA2 axis in breast cancer[J].Reprod Biol Endocrinol,2022,20(1):146-158.
[11] Jin W,Zhang H,Li M,et al.Long noncoding RNA regulator of reprogramming regulates cell growth,metastasis,and cisplatin resistance in gastric cancer via miR-519d-3p/HMGA2 axis[J].Cancer Biother Radiopharm,2023,38(2):122-131.