温肺化纤汤对肺纤维化模型大鼠肺功能及HMGB1/TLR4/NF-κB信号通路的影响

doi:10.3969/j.issn.1006-6233.2025.04.06

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Abstract Objective: To investigate the effects of Wenfei Huaxian decoction (WFHX) on lung function and the high mobility group protein B1 (HMGB1)/Toll-like receptor 4 (TLR4)/nuclear factor-kappa B (NF-κB) signaling pathway in pulmonary fibrosis (PF) model rats. Methods: Idiopathic pulmonary fibrosis (IPF) was induced in rats using bleomycin (BLM).Successfully modeled rats were divided into the Model group,L-WFHX group (0.18 mg/3mL WFHX),M-WFHX group (0.54mg/3mL WFHX),H-WFHX group (0.9mg/3mL WFHX),and WFHX+LPS group (0.9mg/3mL WFHX + 0.4mg/kg LPS),with 10 rats in each group.Another 10 rats were selected as the Control group (induced with physiological saline).The Model and Control groups were orally gavaged (three times daily) and injected (once daily) with an equal amount of physiological saline for 28 consecutive days.Lung function was assessed in all groups.Lung tissue injury was evaluated using HE staining and Masson staining.Immunohistochemistry was used to detect the expression of α-smooth muscle actin (α-SMA) and collagen I in lung tissue.Enzyme-linked immunosorbent assay (ELISA) was used to measure the expression of transforming growth factor-β₁ (TGF-β₁) and interleukin-1β (IL-1β) in lung tissue.Western blot was used to detect the expression of HMGB1,TLR4,and NF-κB proteins in rat lung tissue. Results: The Control group showed clear and intact lung tissue structure with no significant inflammatory cell infiltration or collagen fiber deposition.The Model group exhibited significant lung tissue damage,with extensive inflammatory cell infiltration and collagen deposition.The L-WFHX,M-WFHX,and H-WFHX groups showed significant improvement in lung tissue structure,with reduced inflammatory exudation and collagen deposition.The WFHX+LPS group exhibited aggravated lung tissue damage,with increased inflammatory exudation and collagen deposition.Compared to the Control group,the Model group showed higher FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and lower FVC and MassFVC (P<0.05).Compared to the Model group,the L-WFHX,M-WFHX,and H-WFHX groups showed lower FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and higher FVC and MassFVC (P<0.05).Compared to the H-WFHX group,the WFHX+LPS group showed higher FEV0.4/FVC,Szapiel score,Ashcroft score,α-SMA,collagen I,TGF-β1,IL-1β,HMGB1,TLR4,and NF-κB expression,and lower FVC and MassFVC (P<0.05). Conclusion: WFHX can improve lung function in PF rats,and its mechanism may involve the inhibition of the HMGB1/TLR4/NF-κB signaling pathway.

Key words： Pulmonary fibrosis Wenfei Huaxian decoction High mobility group protein B1 Toll-like receptor 4 Nuclear factor-kappa B

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	TIAN Hui
	FU Taoli
	WANG Limei
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TIAN Hui,FU Taoli,WANG Limei, et al. The Effect of Wenfei Huaxian Decoction on Lung Function and HMGB1/TLR4/NF-κB Signaling Pathway in Pulmonary Fibrosis Model Rats[J]. HeBei Med, 2025, 31(4): 564-570.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.04.06 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I4/564

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