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| Exploring the Protective Effect of Clofibrate on Chronic Renal Failure Rats Based on the PPARα/CPT1A Pathway |
| LIU Lixia, ZHANG Lu, QI Shanshan |
| Shijiazhuang Hospital of Traditional Chinese Medicine, Hebei Shijiazhuang 050000, China |
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Abstract Objective: To investigate the protective effect of clofibrate on chronic renal failure (CRF) rats based on the PPARα/CPT1A pathway. Methods: Sprague-Dawley male rats were randomly assigned into sham surgery (Sham) group,CRF group,clofibrate group,and clofibrate+GW6471 (PPARα inhibitor) group,with 12 rats in each group.A CRF rat model was constructed using 5/6 nephrectomy.After 8 weeks of corresponding drug intervention,the levels of serum SCr,BUN and blood lipid were detected.biochemical method was applied to detect the activities of SOD,GSH-Px,and content of MDA in renal tissue.ELISA method was applied to detect the levels of tumor necrosis factor-α (TNF-α),interleukin-1β,and IL-6 in renal tissue.HE staining was applied to observe pathological changes in kidney tissue.Masson staining was applied to observe renal interstitial fibrosis injury.Western blot was applied to detect the expression of PPARα and CPT1A proteins in the kidneys. Results: Treatment with clofibrate decreased the levels of SCr,BUN,TC,TG,LDL-C in serum,the content of MDA,levels of TNF-α,IL-1β,IL-6,and proportion of interstitial fibrosis area in renal tissue (P<0.05),increased the level of HDL-C in serum,the activities of SOD and GSH-Px,and levels of PPARα and CPT1A proteins in renal tissue (P<0.05).GW6471 could weaken the protective effect of clofibrate on renal injury in CRF rats (P<0.05). Conclusion: Clofibrate may improve lipid metabolism,inhibit oxidative stress and inflammatory response,and alleviate renal injury in CRF rats by activating the PPARα/CPT1A pathway.
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2025, Vol. 31 
