病原体靶向测序技术在持续性炎性反应-免疫抑制-分解代谢综合征老年患者中的诊疗价值

doi:10.3969/j.issn.1006-6233.2025.05.07

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Abstract Objective: To detect pathogens in elderly patients with persistent inflammation immunosuppression catabolism syndrome (PICS) using targeted next-generation sequencing (tNGS) technology, and to evaluate the diagnostic and therapeutic value of tNGS in screening for this disease. Methods: A total of 107 sputum samples from general inflammatory elderly patients and 107 sputum samples from PICS elderly patients admitted to our hospital from Jul. 2023 to Jul. 2024 were collected. tNGS was used to detect pathogens in both groups of patients and to compare the microbial characteristics between the two groups. Results: Using tNGS technology, 45 types of pathogens were identified in the control group, including 20 bacteria, 14 viruses, and 11 fungi; while 48 types of pathogens were identified in the observation group, including 24 bacteria, 12 viruses, and 12 fungi. Among the pathogens isolated using tNGS technology, the top three bacteria detected in the control group were Fusobacterium nucleatum (n=43), Streptococcus anginosus group (n=40), and Haemophilus influenzae (n=34); while in the observation group, they were Fusobacterium nucleatum (n=44), Pseudomonas aeruginosa (n=44), and Acinetobacter baumannii (n=32). The most detected fungi and viruses in both groups were Candida albicans and Epstein-Barr virus, respectively. Conclusion: tNGS is a potential tool for detecting pathogens in elderly patients with PICS, which can be used to detect pathogens in the patients and preliminarily confirm clinical diagnostic candidate markers for PICS.

Key words： Targeted next-generation sequencing technelogy Persistent inflammation immunosuppression catabolism syndrome Pneumonia

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.05.07 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I5/743

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