组织β-catenin c-Myc Wnt1 mRNA表达水平与CIN合并HPV感染患者病理分级及术后复发的关系研究

doi:10.3969/j.issn.1006-6233.2025.06.03

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Abstract Objective: To explore and analyze the expressions of β-catenin, C-Myelocytomatosis viral oncogene homolog (c-Myc) and wingless MMTV integration site family member 1 (Wnt1) in cervical tissues of patients with cervical intraepithelial neoplasia (CIN) combined with human papillomavirus (HPV) infection, and to analyze its relationship with pathological grading and postoperative recurrence. Methods: Totally 92 patients with CIN combined with HPV infection in our hospital from January 2020 to December 2023 were included. The mRNA expression levels of β-catenin, c-Myc and Wnt1 in cervical tissues were determined by real-time fluorescence quantitative PCR, and the association between their expressions and pathological grading and postoperative recurrence was analyzed. Results: The comparison of the expression levels of β-catenin, c-Myc and Wnt1 mRNA in tissues of patients with different CIN grades showed statistically significant differences (P<0.05), and those in CIN grade 3 and CIN grade 2 were higher than those in CIN grade 1, while those in CIN grade 3 were higher than those in CIN grade 2, and the differences were all statistically significant (P<0.05). Spearman correlation showed that the expression levels of β-catenin, c-Myc, and Wnt1 mRNA in tissues were positively correlated with the CIN grade of cervical tissue biopsy (r=0.527, 0.568, 0.574, P<0.05). Ordered Logistic regression showed that the expression levels of tissue β-catenin, c-Myc, and Wnt1 mRNA were risk factors affecting the pathological grade of CIN in cervical tissue biopsy (P<0.05). The expression levels of β-catenin, c-Myc and Wnt1 mRNA in the tissues of patients with postoperative recurrence were higher than those of non-recurrence patients, and the difference was statistically significant (P<0.05). ROC curves revealed that the AUCs of tissue β-catenin, c-Myc and Wnt1 mRNA expression levels in predicting postoperative recurrence were 0.799, 0.849 and 0.795, the sensitivities were 0.941, 0.941 and 0.706, and the specificities were 0.589, 0.649 and 0.821, respectively. Conclusion: The mRNA expression levels of β-catenin, c-Myc and Wnt1 in cervical tissues of CIN patients are related to the pathological grading and postoperative recurrence, and have certain predictive value on postoperative recurrence.

Key words： Cervical intraepithelial neoplasia Human papillomavirus β-catenin C-Myelocytomatosis virus oncogene homolog Wingless MMTV integration site family member 1

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.06.03 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I6/891

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