妊娠期糖尿病孕妇外周血miR-372-3p miR-27a水平与胰岛素抵抗及新生儿结局的相关性研究

doi:10.3969/j.issn.1006-6233.2025.06.18

Abstract
Figure/Table
References
Related Citation (15)

Download: PDF (1268 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective: To observe the association of changes of peripheral blood short-chain non-coding RNA (miRNA)-372-3p and miR-27a with insulin resistance (IR) and neonatal outcomes in patients with gestational diabetes mellitus (GDM). Methods: 102 patients with GDM in the hospital were included from June 2022 to April 2024, and 102 normal pregnant women were selected for control. The levels of miR-372-3p and miR-27a in peripheral blood of the enrolled patients were measured, and the patients were divided into normal outcome group and adverse outcome group according to the neonatal outcomes. The general data and detection information of patients were collected, and ROC curve was drawn to analyze the predictive value of miR-372-3p, miR-27a and insulin resistance on neonatal adverse outcomes of pregnant women with GDM. Results: The peripheral blood miR-372-3p, miR-27a and IR index in GDM group were significantly higher than those in normal pregnant women group the difference was statistically significant (P<0.05). Pearson correlation analysis indicated that the expression levels of miR-372-3p and miR-27a in GDM patients were positively correlated with IR index, and the correlations were statistically significant (r=0.518, 0.606, P<0.05). Linear regression analysis demonstrated that age, body mass index and expression levels of miR-372-3p and miR-27a were influencing factors for IR index in GDM pregnant women, and the associations were statistically significant (P<0.05). The expression levels of miR-372-3p and miR-27a and IR index of GDM pregnant women in neonatal adverse outcome group were higher than those in normal outcome group, and there were statistical differences (P<0.05). ROC curve showed that the AUCs of miR-372-3p, miR-27a and IR in predicting neonatal outcomes of GDM pregnant women were 0.740, 0.806 and 0.669 respectively. Conclusion: The increased expressions of miR-372-3p and miR-27a in peripheral blood of GDM patients promote the risk of IR, and have certain application value on evaluating neonatal outcomes.

Key words： Gestational diabetes mellitus Short-chain non-coding RNA Insulin resistance Neonatal outcomes

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	MA Qianqian
	MA Dan
	CHEN Dongying
	et al

Cite this article:

MA Qianqian,MA Dan,CHEN Dongying, et al. Correlation of Peripheral Blood miR-372-3p and miR-27a Levels with Insulin Resistance and Neonatal Outcome in Pregnant Women with Gestational Diabetes Mellitus[J]. HeBei Med, 2025, 31(6): 973-978.

URL:

http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.06.18 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I6/973

[1] Sweeting A,Wong J,Murphy HR,et al.A clinical update on gestational diabetes mellitus[J].Endocr Rev,2022,43(5):763-793.
[2] Moon JH,Jang HC.Gestational diabetes mellitus:diagnostic approaches and maternal-offspring complications[J].Diabetes Metab,2022,46(1):3-14.
[3] Sert UY,Ozgu-Erdinc AS.Gestational diabetes mellitus screening and diagnosis[J].Adv Exp Med Biol,2021(1307):231-255.
[4] DTuski DF,Wolinska E,Skrzypczak M.Epigenetic changes in gestational diabetes mellitus[J].Int Mol Sci,2021,22(14):7649.
[5] Radojicic O,Dobrijevic Z,Robajac D,et al.Gestational diabetes is associated with an increased expression of miR-27a in peripheral blood mononuclear Cells[J].Mol Diagn Ther,2022,26(4):421-435.
[6] 郭碧莲,刘妍,何荣霞,等.妊娠期糖尿病中miR-372-3p参与调控胰岛素抵抗的关系研究[J].中国妇产科临床杂志,2020,21(5):532-534.
[7] Chatzakis C,Cavoretto P,Sotiriadis A.Gestational diabetes mellitus pharmacological prevention and treatment[J].Curr Pharm Des,2021,27(36):3833-3840.
[8] Zeng Q,Zou D,Liu N,et al.Association of miR-196a2 and miR-27a polymorphisms with gestational diabetes mellitus susceptibility in a Chinese population[J].Front Endocrinol (Lausanne),2023(14):1127336.
[9] Kim Y,Kim OK.Potential roles of adipocyte extracellular vesicle-derived miRNAs in obesity-mediated insulin resistance[J].Adv Nutr,2021,12(2):566-574.
[10] Mir R,Saeedi NH,Jalal MM,et al.Clinical implications of krupple-like transcription factor KLF-14 and certain micro-RNA (miR-27a,miR-196a2,miR-423) gene variations as a risk factor in the genetic predisposition to PCOS[J].Pers Med,2022,12(4):586.
[11] Du J,Zhu Y,Yang X,et al.Berberine attenuates obesity-induced insulin resistance by inhibiting miR-27a secretion[J].Diabet Med,2024,41(7):15319.
[12] Wang D,Zhang X,Li Y,et al.Exercise-induced browning of white adipose tissue and improving skeletal muscle insulin sensitivity in obese/non-obese growing mice:do not neglect exosomal miR-27a[J].Front Nutr,2022(9):940673.
[13] Meng Z,Li F,Wang B.miR-372-3p is a potential diagnostic factor for diabetic nephropathy and modulates high glucose-induced glomerular endothelial cell dysfunction via targeting fibroblast growth factor-16[J].Arch Med Sci,2019,19(3):703-716.