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| Effect of Ligustilide on Hypoxia/Reoxygenation Induced Myocardial Cell Injury by Adjusting the Notch1/Hes1 Signaling Pathway |
| LI Lihua, ZHANG Ning, XU Ying, et al |
| Baoding First Central Hospital, Hebei Baoding 071000, China |
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Abstract Objective: To explore the effects of ligustilide on hypoxia/reoxygenation induced myocardial cell injury by adjusting Notch1/Hes1 signaling pathway. Methods: H9C2 cardiomyocytes were used as the research object and grouped: cells with normal culture were labeled as the normal group, hypoxia/reoxygenation induction as the hypoxia/reoxygenation group, hypoxia/reoxygenation induction+10μmoL/L ligustilide as the hypoxia/reoxygenation+low-dose ligustilide group, hypoxia/reoxygenation induction+20μmoL/L ligustilide as the hypoxia/reoxygenation+medium-dose ligustilide group, hypoxia/reoxygenation induction+40μmoL/L ligustilide as the hypoxia/reoxygenation+high-dose ligustilide group, and hypoxia/reoxygenation induction+40μmoL/L ligustilide+5 mg/L Notch activator Jagged1 as the hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group. MTT, flow cytometry, ELISA, and Western blot were used to detect cell proliferation, apoptosis, LDH, CK, AST, IL-18, IL-1β levels, and the Notch1/Hes1 signaling pathway related proteins. Results: The hypoxia/reoxygenation group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the normal group (P<0.05). The hypoxia/reoxygenation+low, medium, and high-dose ligustilide groups showed increased OD value, and decreased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation group (P<0.05). The hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation+high-dose ligustilide group. Conclusion: The improvement effect of ligustilide on hypoxia/reoxygenation induced myocardial cell injury may be achieved by inhibiting Notch1/Hes1 signaling pathway.
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2025, Vol. 31 
