宫颈癌及癌前病变中的HPV-16整合人宿主基因组的发生情况及其在宫颈癌筛查中的应用

doi:10.3969/j.issn.1006-6233.2017.11.003

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Abstract Objective: To investigate the prevalence of physical state of HVP-16 DNA in cervical and cervical precancerous carcinoma. Methods: Multiplex PCR was adopted to detect the physical state of HPV in samples from 252 patients with cervical carcinoma, including 48 samples of cervical cancer, 204 cervical intraepithelial(CIN)(125 CIN I,46 CIN Ⅱ and 33 CIN Ⅲ)and 20 normal samples from the subjects with hysteromyoma undergoing hysterectomy ,respectively. Results: Not only patients with cervical cancer but also patients with precancerous were found to have integrated infection with HPV-16 and those with cervical cancer have the highest integration rate. With the elevating of the grades of CIN, the integrated infection rates of HVP-16 was significantly elevated, the difference was statistically significant (χ²=49.7359,12.6033, P<0.01). Conclusion: Among the patients with HPV-16 infection,the integrated state of HVP-16 is positively correlated with the severity of cervical lesions. Combined HVP typing test and detection of integrated viral contribute to predicting the prognosis of patients with cervical precancerous lesions and increasing the accuracy of screening cervical cancer on the basis of HPV DNA detection.

Key words： Cervical cancer Precancerous tissues Human papillomavirus Integration

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	WANG Xia

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WANG Xia. Integrated State of HPV-16 in Precancerous Tissues and CervicalCancer and Application in Cervical Cancer Screening[J]. HeBei Med, 2017, 23(11): 1768-1771.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2017.11.003 OR http://www.hbyxzzs.cn/EN/Y2017/V23/I11/1768

[1] 凌王芳,王璐璐,蒋玲玲,等.人乳头瘤病毒感染与宫颈病变的关系研究[J].实用预防医学,2016,23(4):464~466.
[2] Singh MP, Gupta N, Deepak T, et al. Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed paraffin-embedded cervical tissues[J].Indian Med Microbiol, 2017,35(1):113~115.
[3] Tan SC, Ismail MP, Duski DR, et al. Identification of optimal reference genes for normalization of RT-qPCR data in cancerous and non-cancerous tissues of human uterine cervix[J].Cancer Invest, 2017,35(3):163~173.
[4] 韩会娟.HPV 16/18在宫颈癌组织中的表达及其临床意义[J].江苏医药,2014,40(9):1052~1054.
[5] 张乔,王诗卓,魏恒,等.持续感染状态的HPV-16及HPV-58亚型在宫颈上皮内瘤变及宫颈癌中的分布及意义[J].中国妇幼保健,2012,27(23):3564~3567.
[6] Wang W, Xia X, Wang S, et al. Oncolytic adenovirus armed with human papillomavirus E2 gene in combination with radiation demonstrates synergistic enhancements of antitumor efficacy[J]. Cancer Gene Ther, 2011, 18(11): 825~836.
[7] 魏文斐,苏桂栋,吴兰芳,等.宫颈癌与癌前病变组织中HPV-16的整合感染状态[J].南方医科大学学报,2015,(1):47~50.
[8] Gadducci A, Barsotti C, Laliscia C, et al. Dose-dense paclitaxel- and carboplatin-based neoadjuvant chemotherapy followed by surgery or concurrent chemo-radiotherapy in cervical cancer: a preliminary analysis[J].Anticancer Res, 2017,37(3):1249~1255.
[9] Singh MP, Gupta N, Deepak T, et al. Multiplex polymerase chain reaction for the detection of high-risk-human papillomavirus types in formalin-fixed paraffin-embedded cervical tissues[J].Indian Med Microbiol, 2017,35(1):113~115.
[10] 马春华,吐尔逊阿衣·买买提明,王琳,等.高危型 HPV 基因型检测在新疆宫颈癌筛查中的临床应用价值[J].中国生育健康杂志,2016,27(5):432~437.
[11] 谢月英.宫颈人乳头瘤病毒亚型分布及其与宫颈癌的关系[J].中国妇幼保健,2015,30(25):4275~4277.