Abstract：Objective: To investigate the relationship between changes in serum levels of heat shock protein 70 (HSP70) and thioredoxin 1 (TRX-1) with the progression of chronic atrophic gastritis (CAG) and Helicobacter pylori (Hp) infection. Methods: From April 2022 to June 2024,165 patients with CAG admitted to the Gastroenterology Department of our hospital were included as the research subjects.According to the presence or absence of Hp infection in CAG patients,they were separated into CAG patients with Hp infection (Hp group,n=95) and CAG patients without Hp infection (non-Hp group,n=70).The serum levels of HSP70 and TRX-1 were detected by ELISA.The correlation between HSP70,TRX-1 levels with pepsinogen Ⅰ (PG-Ⅰ),pepsinogen Ⅱ (PG-Ⅱ) (Pearson method),and clinical symptom score (Spearman method) was analyzed.Multivariate logistic regression was applied to analyze the influencing factors of Hp infection.ROC curve was applied to evaluate the diagnostic value of HSP70 and TRX-1 levels for Hp infection. Results: The proportion of people with unreasonable diet,clinical symptom scores,and expression levels of HSP70 and TRX-1 in the Hp group were significantly higher than those in the non-Hp group,while the levels of PG-Ⅰ and PG-Ⅱ were lower than those in the non-Hp group (t=5.163,15.129,9.672,5.632,13.561,8.347,all P<0.05).The levels of HSP70 and TRX-1 were significantly negatively correlated with the levels of PG-I and PG-Ⅱ (r=-0.687,-0.696,-0.742,-0.651,all P<0.05),and significantly positively correlated with clinical symptom scores (r=0.716,0.668,all P<0.05).The expression levels of serum HSP70 and TRX-1 in the mild,moderate,and severe groups increased successively (F=17.986,38.924,all P<0.05).The proportions of patients with moderate to severe infiltration,metaplastic atrophy,moderate to severe inflammatory response,moderate to severe atrophy,and moderate to severe intestinal metaplasia in the Hp group were higher than those in the non-Hp group (P<0.05).PG-I and PG-Ⅱ were protective factors for Hp infection in CAG patients (OR=0.483,0.585,all P<0.05),while HSP70 and TRX-1 were risk factors for Hp infection in CAG patients (OR=4.562,5.138,all P<0.05).The AUCs of HSP70 and TRX-1 levels for diagnosing Hp infection in CAG patients were 0.833 and 0.722,respectively.When the two were combined for diagnosing Hp infection,the AUC increased to 0.861,with the highest sensitivity.The AUC of the combined diagnosis of Hp infection by HSP70 and TRX-1 was higher than that of the single diagnosis by HSP70 (Z=2.034,P<0.05) and TRX-1 (Z=2.847,P<0.05). Conclusion: Serum HSP70 and TRX-1 are upregulated in patients with CAG,they are closely related to the development of the disease and Hp infection,and the combination of the two has a high diagnostic value for Hp infection in CAG patients.
康雪兰, 胡晓翠, 汝童, 王裕麟. 血清HSP70 TRX-1水平变化与慢性萎缩性胃炎患者Hp感染的关系[J]. 河北医学, 2025, 31(4): 676-682.
KANG Xuelan, HU Xiaocui, RU Tong, et al. Relationship Between Serum HSP70 and TRX-1 Levels and Hp Infection in Patients with Chronic Atrophic Gastritis. HeBei Med, 2025, 31(4): 676-682.
2025, Vol. 31 
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