Abstract：Objective: To investigate the mechanism of astragaloside IV in regulating the GSK-3β/BMP2 signaling pathway on tooth movement and periodontal tissue in orthodontic rats. Methods: Sixty SPF male SD rats were selected,with 18 rats assigned to the blank control group and the remaining 42 rats used to establish orthodontic models.Among the 39 successfully modeled rats,they were randomly divided into a model group (15 rats),an astragaloside IV group (12rats),and an astragaloside IV + CHIR-99021 group (12rats).The blank control group and model group were administered 10mg/kg of 0.9% sodium chloride solution via intragastric gavage and intraperitoneal injection.After successful modeling,the astragaloside IV group received 80mg/kg astragaloside IV via intragastric gavage and 10mL/kg of 0.9% sodium chloride solution via intraperitoneal injection.The astragaloside IV + CHIR-99021 group received 80mg/kg astragaloside IV and 30mg/kg CHIR-99021 via intragastric gavage.Periodontal histopathological changes,alveolar bone resorption,serum inflammatory factors,osteoclast count,periodontal index,tooth movement distance,alveolar bone injury-related indicators,serum oxidative stress markers,and GSK-3β/BMP2 signaling pathway-related protein expression in periodontal tissue were compared among the groups. Results: Compared with the blank control group,the astragaloside IV + CHIR-99021 group and model group showed higher levels of IL-17,reactive oxygen species,TNF-α,PD,IL-6,malondialdehyde,osteoclast count,PLI,alveolar bone resorption,and SBI,while BMP2,Tb.Th,SOD,GSK-3β,BV/TV,tooth movement distance (7d,14d,21d),Tb.N,and glutathione levels were lower (P<0.05).Compared with the model group,the astragaloside IV group showed reduced levels of IL-17,malondialdehyde,PD,reactive oxygen species,IL-6,alveolar bone resorption,PLI,TNF-α,osteoclast count,and SBI,while Tb.Th,GSK-3β,BV/TV,SOD,BMP2,Tb.N,tooth movement distance (7d,14d,21d),and glutathione levels were increased (P<0.05).Compared with the astragaloside IV group,the astragaloside IV + CHIR-99021 group showed decreased Tb.Th,glutathione,BMP2,SOD,tooth movement distance (7d,14d,21d),BV/TV,GSK-3β,and Tb.N,while PD,IL-17,osteoclast count,reactive oxygen species,PLI,TNF-α,alveolar bone resorption,malondialdehyde,IL-6,and SBI levels were increased (P<0.05). Conclusion: Astragaloside IV can inhibit oxidative stress and alveolar bone resorption,reduce osteoclast formation,and promote tooth movement in rats,potentially through activation of the GSK-3β/BMP2 signaling pathway.
李培培, 娄会杰, 杨大鹏. 黄芪甲苷调节GSK-3β/BMP2信号通路对正畸大鼠牙齿移动和牙周组织的作用机制[J]. 河北医学, 2025, 31(4): 583-591.
LI Peipei, LOU Huijie, YANG Dapeng. Mechanism of Astragaloside IV in Regulating the GSK-3 β/BMP2 Signaling Pathway on Tooth Movement and Periodontal Tissue in Orthodontic Rats. HeBei Med, 2025, 31(4): 583-591.
2025, Vol. 31 
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