Abstract：Objective: To investigate the impacts of long non-coding RNA (LncRNA) non-coding RNA activated by DNA damage (NORAD) on migration and invasion of acute myeloid leukemia (AML) cells by adjusting microRNA-136-5p (miR-136-5p)/chromobox protein homolog 4 (CBX4) axis. Methods: Human AML cell line KG1a was used as the research subject and divided into control group, sh-NC group, sh-NORAD group, sh-NORAD+NC-inhibitor group, and sh-NORAD+miR-136-5p-inhibitor group, with six replicates in each group. The expressions of LncRNA NORAD, miR-136-5p, and CBX4 mRNA in KG1a cell lysate were detected by qRT-PCR. Plate cloning, scratch assay, and Transwell assay were performed to detect the impacts of knocking down LncRNA NORAD on proliferation, migration, and invasion of KG1a cells. Moreover, Western blot was used to detect protein expression. Results: Compared with sh-NC group and control group, the clone number, scratch healing rate, invasion number, LncRNA NORAD, CBX4 mRNA and protein, MMP-9 were decreased in sh-NORAD group, and the expression of miR-136-5p was increased (P<0.05). Compared with sh-NORAD group and sh-NORAD+NC-inhibitor group, the clone number, scratch healing rate, invasion number, CBX4 mRNA and protein and MMP-9 of sh-NORAD+miR-136-5p-inhibitor group were increased. The expression of miR-136-5p was decreased (P<0.05). LncRNA NORAD could target the negative regulation of miR-136-5p, and miR-136-5p could target the negative regulation of CBX4. Conclusion: LncRNA NORAD can regulate the miR-136-5p/CBX4 axis to promote migration and invasion of AML cells.
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2025, Vol. 31 
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