Abstract：Objective: To explore the expression of miR-106b-5p in colon cancer tissues and its influences on biological function of human colon cancer LOVO/HCT116 cells. Methods: The expression of has-miR-106b-5p in human colon cancer tissues and para-carcinoma tissues was detected by real-time fluorescent quantitative PCR (RT-PCR). Taking colon cancer LOVO/HCT116 cells as the study background, cell lines with miR-106b-5p overexpression and their negative controls (miR-106b-5p mimic group, mimic NC group) were constructed by miR-106b-5p-mimic and mimic NC, and they were verified by RT-PCR. The cells proliferation, cycle distribution, apoptosis, migration and invasion after transfection were detected by CCK8, flow cytometry and Tanswell assay, respectively. The expressions of related proteins were detected by Western blot. Results: The expression of has-miR-106b-5p in human colon cancer tissues was lower than that in para-carcinoma tissues (t=101.720, P<0.001). After transfection with miR-106b-5p, expression of has miR-106b-5p in LOVO/HCT116 cells in miR-106b-5p mimic group was higher than that in mimic-NC group (P<0.05). Compared with mimic group, cell proliferation rates were decreased in miR-106b-5p mimic group at 24, 48, 72, 96 and 120h after transfection (P<0.05), proportion of G0/G1 stage and apoptosis rate were increased, proportion of S stage and number of cells migration and invasion cells were decreased (P<0.05), expressions of vimentin (Vimentin), phosphorylated extracellular regulated kinase 1/2 (p-ERK1/2) and phosphorylated protein kinase B1/2/3 (p-AKT1/2/3) proteins were decreased (P<0.05), and expressions of E-cadherin and cleaved cysteine proteinase 3 (cleaved-caspase3) proteins were increased (P<0.05). Conclusion: The expression of miR-106b-5p is down-regulated in colon cancer tissues. Overexpression of miR-106b-5p can inhibit proliferation, migration and invasion of human colon cancer LOVO/HCT116 cells, and promote apoptosis, which may be related to the regulation of AKT and ERK signaling pathways.
马海林, 蒋升, 马志萍. miR-106b-5p在结肠癌组织中的表达及其对人结肠癌LOVO/HCT116细胞生物学功能的影响[J]. 河北医学, 2025, 31(8): 1269-1275.
MA Hailin, JIANG Sheng, MA Zhiping. Expression of miR-106b-5p in Colon Cancer Tissues and its Influences on Biological Function of Human Colon Cancer LOVO/HCT116 Cells. HeBei Med, 2025, 31(8): 1269-1275.
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2025, Vol. 31 
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