Abstract：Objective: To investigate the effect of arctigenin (ARC) on hypoxia/reoxygenation (H/R) - induced apoptosis of human renal proximal tubular epithelial HK-2 cells by regulating the stromal cell-derived factor-1 (SDF-1)/CXCR4 signaling pathway. Methods: HK-2 cells were cultured in vitro and separated into Control group, Model group, L-ARC, M-ARC, H-ARC group (5, 10, 20 μmol/L ARC), and AMD group (20 μmol/L ARC+5 μmol/L SDF-1/CXCR4 signaling pathway inhibitor AMD3100) randomly. Except for the normal culture of the Control group, H/R cell models were established in all other groups. MTT and clone formation experiments were applied to detect the proliferation of cells in each group. DCFH-DA fluorescent probe was applied to detect the level of reactive oxygen species (ROS). The reagent kit was used to detect changes in the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) of cells in each group. Flow cytometry was applied to detect apoptosis of cells in various groups. Western blotting (WB) was applied to detect the expression of apoptosis related proteins (Bcl-2, Bax) and SDF-1/CXCR4 signaling pathway related proteins (SDF-1, CXCR4) of cells in each group. Results: Compared with the Control group, the proliferation rate, clone number, SOD, and Bcl-2 protein expression of HK-2 cells in the Model group decreased, while the apoptosis rate, ROS, MDA, Bax, SDF-1, and CXCR4 protein expression increased (P<0.05). Compared with the Model group, the proliferation rate, clone number, SOD, Bcl-2, SDF-1, and CXCR4 protein expression of cells in the L-ARC group, M-ARC group, and H-ARC group increased, while the apoptosis rate, ROS, MDA, and Bax protein expression decreased (P<0.05). Compared with the H-ARC group, the proliferation rate, clone number, SOD, Bcl-2, SDF-1, and CXCR4 protein expression in AMD group reduced, while the apoptosis rate, ROS, MDA, and Bax protein expression increased (P<0.05). Conclusion: ARC may alleviate oxidative stress response, inhibit H/R-induced HK-2 cell apoptosis, promote cell proliferation, and improve renal proximal tubular epithelial cell damage by promoting the activation of the SDF-1/CXCR4 signaling pathway.
李兰梅, 白伟伟, 陈娜, 孙绍婷, 王萌, 张明, 李亚芬. 牛蒡子苷元调节SDF-1/CXCR4信号通路对缺氧复氧诱导的HK-2细胞凋亡的影响[J]. 河北医学, 2025, 31(10): 1607-1612.
LI Lanmei, BAI Weiwei, CHEN Na, et al. Effect of Arctigenin on Hypoxia/Reoxygenation-Induced Apoptosis of HK-2 Cells by Regulating the SDF-1/CXCR4 Signaling Pathway. HeBei Med, 2025, 31(10): 1607-1612.
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2025, Vol. 31 
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