Abstract：Objective: To investigate the effect of microRNA-30a-5p (miR-30a-5p) on vascular calcification in chronic kidney disease (CKD) rats by targeting Runt-related transcription factor-2 (Runx2). Methods: CKD model rats were constructed and randomly grouped into model group, miR-30a-5p negative control group (miR-30a-5p-NC group), miR-30a-5p mimic group (miR-30a-5p mimics group), miR-30a-5p mimic+empty plasmid group (miR-30a-5p+pcDNA-NC group), and miR-30a-5p mimic+Runx2 overexpression group (miR-30a-5p+Runx2 group). Healthy rats were made the control group. RT-qPCR was used to detect the miR-30a-5p and Runx2 mRNA in the aorta. The 24-hour urine protein content (reagent kit method), serum creatinine (SCr), calcium (Ca), phosphorus (P), blood urea nitrogen (BUN), and serum cystatin C (CysC) in rats (biochemical analyzer); the pathological changes in renal tissue (HE staining method); aortic calcified nodules (Alizarin Red staining); calcium content and ALP activity of vascular wall (kit method); the positive expression of α-SMA and OPN in the aorta (immunohistochemistry), and the protein contents of Runx2, BMP2, and OCN (Western blot) were measured. Dual luciferase was used to determine the targeting relationship between miR-30a-5p and Runx2. Results: The kidney tissue of rats in the model group showed conspicuous pathological changes and aggravated renal aortic calcification, and the 24-hour urinary protein, renal function indexes, Ca content, ALP activity, OPN positive expression, and Runx2, BMP2, OCN protein contents in the aorta were conspicuously increased, while the miR-30a-5p and α-SMA positive expression in the aorta were conspicuously decreased (P<0.05). Compared with the miR-30a-5p-NC group, the miR-30a-5p mimics group showed reduced renal tissue damage and renal aortic calcification in rats, and the 24-hour urinary protein, renal function indexes, Ca content, ALP activity, OPN positive expression, and Runx2, BMP2, OCN protein contents in the aorta were conspicuously decreased, while the miR-30a-5p and α-SMA positive expression in the aorta were conspicuously increased (P<0.05). Compared with the miR-30a-5p+pcDNA-NC group, the miR-30a-5p+Runx2 group showed severe renal tissue damage, aggravated renal aortic calcification, decreased renal function, and the α-SMA positive expression in the aorta was conspicuously reduced, while the OPN positive expression and Runx2, BMP2, and OCN protein contents were conspicuously increased (P<0.05). The dual luciferase reporter gene experiment demonstrated a targeted relationship between miR-30a-5p and Runx2. Conclusion: MiR-30a-5p may enhance renal function, improve kidney injury, and inhibit vascular calcification in CKD rats by targeting Runx2.
于沛, 赵小云, 李琴, 张恒. miR-30a-5p靶向Runx2对慢性肾病大鼠血管钙化的影响[J]. 河北医学, 2025, 31(10): 1632-1639.
YU Pei, et al. The Effect of miR-30a-5p on Vascular Calcification in Rats with Chronic Kidney Disease by Targeting Runx2. HeBei Med, 2025, 31(10): 1632-1639.
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2025, Vol. 31 
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