针刺结合黄芪建中汤调节PI3K/AKT信号通路对胃溃疡大鼠表皮生长因子胃黏膜功能的影响

doi:10.3969/j.issn.1006-6233.2025.12.08

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摘要 目的: 探讨针刺结合黄芪建中汤对胃溃疡(GU)大鼠表皮生长因子(EGF)、胃黏膜功能的改善作用,并基于磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(AKT)信号通路探讨其作用机制。方法: 采用灌胃冰乙酸建立GU大鼠模型。将大鼠随机分为对照组(正常大鼠)、模型组、黄芪建中汤组(6.8g/kg黄芪建中汤,灌胃)、电针组(电针刺激足三里、中脘穴)、联合组(6.8g/kg黄芪建中汤灌胃,针刺刺激足三里、中脘穴),每组15只。治疗2周后 ,检测各组大鼠的胃黏膜溃疡面积和溃疡指数;苏木素伊红(HE)染色观察胃黏膜病理组织学变化;ELISA试剂盒检测血清胃泌素(GAS)、生长激素抑制素(SS)、胃动素(MTL)和肿瘤坏死因子α(TNF-α)、白介素6(IL-6)、前列腺素E2(PGE2)以及表皮生长因子(EGF)水平;Western Blot检测PI3K/AKT通路相关蛋白表达水平。结果: 相较于对照组,模型组大鼠胃黏膜溃疡,腺体减少,有大量炎性细胞浸润,SS、PGE2、EGF、p-PI3K、p-AKT水平显著降低(P<0.05),溃疡面积、溃疡指数,GAS、MTL、TNF-α、IL-6水平显著增加(P<0.05);相较于模型组,黄芪建中汤组、针刺组、联合组大鼠胃黏膜损伤有明显改善,溃疡面积、溃疡指数显著降低(P<0.05),SS、PGE2、EGF、p-PI3K、p-AKT水平显著增加(P<0.05),GAS、MTL、TNF-α、IL-6水平显著降低(P<0.05);相较于单独的黄芪建中汤组、针刺组,联合组大鼠溃疡面积、溃疡指数显著降低(P<0.05),SS、PGE2、EGF、p-PI3K、p-AKT水平显著增加(P<0.05),GAS、MTL、TNF-α、IL-6水平显著降低(P<0.05)。结论: 黄芪建中汤联合针刺治疗能够协同增效,通过抑制炎症反应,促进胃黏膜修复和愈合,改善GU大鼠胃黏膜功能,所涉及作用机制可能与激活PI3K/AKT信号通路有关。

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	武九龙
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关键词 ：胃溃疡, 黄芪建中汤, 电针, PI3K/AKT信号通路, 表皮生长因子, 胃黏膜功能

Abstract：Objective: To explore the effect of acupuncture combined with Huangqi Jianzhong decoction on improving epidermal growth factor (EGF) and gastric mucosal function in rats with gastric ulcer (GU), and to explore its mechanism based on phosphatidylinositol 3- kinase (PI3K)/ protein kinase B(AKT) signaling pathway. Methods: GU rat model was established by intragastric glacial acetic acid. Rats were randomly divided into control group (normal rats), model group, Huangqi Jianzhong decoction group (6.8g/kg Huangqi Jianzhong decoction by gavage), electroacupuncture group (electroacupuncture stimulating Zusanli and Zhongwan points) and combined group (6.8g/kg Huangqi Jianzhong decoction by gavage and acupuncture stimulating Zusanli and Zhongwan points), with 15 rats in each group. After 2 weeks of treatment, the gastric ulcer area and ulcer index of rats in each group were detected. HE matoxylin eosin (He) staining was used to observe the pathological changes of gastric mucosa. Serum GAStrin (gas), growth hormone inhibin (SS), motilin (MTL), tumor necrosis factor α(TNF-α), interleukin 6(IL-6), prostaglandin E2(PGE2) and epidermal growth factor (EGF) were detected by ELISA kit. Western Blot was used to detect the expression level of PI3K/AKT pathway related proteins. Results: Compared with the control group, the model group had gastric mucosa ulcer, fewer glands, and a large number of inflammatory cells infiltrated, ulcer area and ulcer index were significantly increased, while, SS, PGE2, EGF, p-PI3K and p-Akt levels were significantly decreased (P<0.05), and the levels of GAS, MTL, TNF-α and IL-6 were significantly increased (P<0.05). Compared with model group, the gastric mucosal injury of rats in Huangqi Jianzhong Decoction group, acupuncture group and combined group was significantly improved, the ulcer area and ulcer index were significantly decreased, SS, PGE2, EGF, p-PI3K and p-Akt levels were significantly increased (P<0.05), and the levels of GAS, MTL, TNF-α and IL-6 were significantly decreased (P<0.05). Compared with Huangqi Jianzhong Decoction group and acupuncture group, the ulcer area and ulcer index were significantly decreased, SS, PGE2, EGF, p-PI3K and p-Akt levels of rats in combined group were significantly increased (P<0.05), while the levels of GAS, MTL, TNF-α and IL-6 were significantly decreased (P<0.05). Conclusion: Huangqi Jianzhong decoction combined with acupuncture can promote the repair and healing of gastric mucosa and improve the gastric mucosal function in GU rats by inhibiting inflammatory reaction, and the mechanism involved may be related to the activation of PI3K/AKT signaling pathway.

Key words： Gastric ulcer Huangqi Jianzhong decoction Acusector PI3K/AKT signaling pathway Epidermal growth factor Gastric mucosal function

基金资助:江苏省优势学科建设工程项目,(编号:YSHL2201-191)

通讯作者: 范钦梅

引用本文:

武九龙, 范钦梅. 针刺结合黄芪建中汤调节PI3K/AKT信号通路对胃溃疡大鼠表皮生长因子胃黏膜功能的影响[J]. 河北医学, 2025, 31(12): 1983-1989.
WU Jiulong, et al. Effects of Acupuncture Combined with Huangqi Jianzhong Decoction to Regulate PI3K/AKT Signaling Pathway on Epidermal Growth Factor and Gastric Mucosa Function in Rats with Gastric Ulcer. HeBei Med, 2025, 31(12): 1983-1989.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.12.08 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I12/1983

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