Abstract：Objective: To explore the effects of salidroside (Sal) on endothelial dysfunction in hypertensive disorder of pregnancy (HDP) rats based on AMPK-eNOS pathway. Methods: Rat models of HDP were constructed. The successfully modeled rats were randomly classified into HDP group, Sal-L group, Sal-H group, and Sal-H+Compound C (AMPK inhibitor) group, with 12 rats in each group. Another 12 normal healthy rats were made the control group. Blood pressure and 24h urinary protein were measured before and after administration. ELISA was performed to measure serum endothelial function biomarkers and inflammatory factors. HE staining was performed to observe pathological changes in kidney tissue and placenta. Immunohistochemistry was used to detect sFLT-1 and PLGF. Western blot was used to measure the AMPK-eNOS pathway related proteins. Results: For the control group, the HDP group showed aggravated renal tissue pathological damage, increased glomerular edema, and even sclerosis, dilation of renal cysts, thickening of basement membrane, shrinkage of placental structure, reduction of placental villi, and even fibrin like necrosis, and there was an increase in vacuolated cells in the trophoblast layer and infiltration of inflammatory cells. The mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1 expression increased, while the NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression decreased (P<0.05). The pathological damage of renal tissue and placental tissue in the Sal-L group and Sal-H group was alleviated compared with the HDP group, with a decrease of the mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1, and an increase in the NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression (P<0.05). The Sal-H+Compound C group showed an increase in mean arterial pressure, 24h UTP, ET-1, TXB2, TNF-α, IL-6, IL-17 levels, and sFLT-1 expression, and a decrease in NO level and PLGF, p-AMPK/AMPK, p-AKT/AKT, and p-eNOS/eNOS expression than Sal-H group (P<0.05). Conclusion: Salidroside can improve endothelial dysfunction in HDP rats and is associated with the activation of AMPK-eNOS pathway.
郑婷华, 宋慧珍, 王丽丽, 宋志慧. 基于AMPK-eNOS通路探讨红景天苷对妊娠高血压大鼠内皮功能障碍的影响[J]. 河北医学, 2025, 31(11): 1761-1766.
ZHENG Tinghua, SONG Huizhen, WANG Lili, et al. Exploring the Effects of Salidroside on Endothelial Dysfunction in Rats with Hypertensive Disorder of Pregnancy Based on AMPK-eNOS Pathway. HeBei Med, 2025, 31(11): 1761-1766.
[1] Wu P,Green M,Myers JE.Hypertensive disorders of pregnancy[J].BMJ,2023,381(1):653-668. [2] Cifkova R.Hypertension in pregnancy:a diagnostic and therapeutic overviewv[J].High Blood Press Cardiovasc Prev,2023,30(4):289-303. [3] Zhu J,Li L,Luan Y,et al.Salidroside pre-treatment inhibits hypertensive renal injury and fibrosis through inhibiting Wnt/β-catenin pathway[J].Dose Response,2024,22(4):1-13. [4] Cheng L,Wang L,Guo M,et al.Clinically relevant high levels of human C-reactive protein induces endothelial dysfunction and hypertension by inhibiting the AMPK-eNOS axis[J].Clin Sci (Lond),2020,134(13):1805-1819. [5] Ribeiro VR,Romao-Veiga M,Nunes PR,et al.Vitamin D modulates the transcription factors of T cell subsets to anti-inflammatory and regulatory profiles in preeclampsia[J].Int Immunopharmacol,2021,101(2):366-376. [6] Wang S,Zhu C,Zhang S,et al.The Combination of gastrodin and gallic acid synergistically attenuates AngⅡ-induced apoptosis and inflammation via regulation of sphingolipid metabolism[J].Inflamm Res,2024,17(1):6971-6988. [7] Wu J,Zhang D,Hu L,et al.Paeoniflorin alleviates NG-nitro-L-arginine methyl ester (L-NAME)-induced gestational hypertension and upregulates silent information regulator 2 related enzyme 1 (SIRT1) to reduce H2O2-induced endothelial cell damage[J].Bioengineered,2022,13(2):2248-2258. [8] Sun Q,Zhang L,Huang X,et al.Salidroside prevents gestational hypertension-induced impairment of offspring learning and memory via Wnt/Skp2 pathway[J].Neurosci Lett,2024,832(1):787-797. [9] Li L,Yang Y,Zhang H,et al.Salidroside ameliorated intermittent hypoxia-aggravated endothelial barrier disruption and atherosclerosis via the cAMP/PKA/RhoA signaling pathway[J].Front Pharmacol,2021,12(1):922-933. [10] Li J,Zhang Z,Zhu C,et al.Salidroside enhances NO bioavailability and modulates arginine metabolism to alleviate pulmonary arterial hypertension[J].Eur Med Res,2024,29(1):423-436. [11] Chen S,Xie JD,Xie MT,et al.Przewaquinone A inhibits Angiotensin Ⅱ-induced endothelial diastolic dysfunction activation of AMPK[J].Phytomedicine,2024,133(1):885-895. [12] Yu Y,Xu LS,Wu Y,et al.The antihypertensive effect of MK on spontaneously hypertensive rats through the AMPK/Akt/eNOS/NO and ERK1/2/Cx43 signaling pathways[J].Hypertens Res,2021,44(7):781-790.
2025, Vol. 31 
冀公网安备13080202000786号