Abstract：Objective: To explore the effect of sesamol (SE) on skin damage in chronic eczema (CE) rats by adjusting the mitogen-activated protein kinase (MAPK)/nuclear factor-κB (NF-κB) signaling pathway. Methods: A CE model rat was established, and the successfully modeled rats were stochastically divided into a CE group, a SE-L group, a SE-H group (external application of 0.7% and 2.1% SE dissolved in 0.1% CMC-Na solution), and a SE-H+Anisomycin group (external application of 2.1% SE+1.4mg/mL MAPK activator Anisomycin dissolved in anhydrous ethanol), each with 12 rats. Another 12 normal rats were stochastically selected as NC group (shaved only, without any other treatment). The rats in NC and CE groups were treated with equal doses of 0.1% CMC-Na and anhydrous ethanol for 10 days. ELISA method was used to detect serum IgE, His, IL-1β, and TNF-α. HE staining and methylamine blue staining were used to observe pathological changes and the number of mast cells in skin lesions. Immunohistochemistry was used to detect the Tryptase protein in skin lesions. Moreover, Western blot was used to detect the protein levels of the MAPK/NF-κB signaling pathway. Results: Compared with the control group, the epidermis and spinous layer of rats in CE group were thickened, and a large amount of inflammatory cell infiltration was observed. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were prominently increased (P<0.05), while cytoplasmic NF-κBp65 protein expression was prominently decreased (P<0.05). Compared with CE group, SE-L group and SE-H group showed reduced thickening of the epidermis and spinous layer of the skin lesions in rats. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were conspicuously decreased (P<0.05), while cytoplasmic NF-κBp65 protein expression was conspicuously increased (P<0.05). Compared with SE-H group, H-SE+Anisomycin group showed increased damage to the skin lesion tissue. The EASI score, serum IgE, His, IL-1β, TNF-α levels, number of mast cells, Tryptase, p-ERK/ERK, p-JNK/JNK, p-p38MARK/p38MARK, p-NF-κBp65/NF-κBp65 protein expression, and nuclear NF-κBp65 protein expression were conspicuously increased (P<0.05), while cytoplasmic NF-κBp65 protein expression was conspicuously decreased (P<0.05). Conclusion: SE may improve skin damage in CE rats by inhibiting the MAPK/NF-κB signaling pathway.
王丽, 周晓丽, 孙振慧, 丁琪. 芝麻酚调节MAPK/NF-κB信号通路对慢性湿疹大鼠皮肤损伤的影响[J]. 河北医学, 2025, 31(11): 1767-1772.
WANG Li, ZHOU Xiaoli, SUN Zhenhui, et al. Effect of Sesamol on Skin Damage in Chronic Eczema Rats by Adjusting the MAPK/NF-κB Signaling Pathway. HeBei Med, 2025, 31(11): 1767-1772.
2025, Vol. 31 
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