益气化瘀方调节TLR4/NF-κB通路介导细胞焦亡改善大鼠子宫内膜炎

doi:10.3969/j.issn.1006-6233.2025.12.06

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摘要 目的: 探究益气化瘀方调节Toll样受体4/核因子κB(TLR4/NF-κB)通路介导细胞焦亡改善大鼠子宫内膜炎。方法: 选取55只SPF级大鼠为实验对象,随机数字表法分为五组,每组11只,通过子宫内膜炎建模处理后,分组为11只空白组,模型组、中药对照组、益气化瘀方组、益气化瘀方+TAK242组,每组各10只。对比各组大鼠炎症因子水平、氧化应激、病理评分及TLR4、NF-κB蛋白等。结果: 相比空白组,模型组、中药对照组、益气化瘀方+TAK242组病理综合评分、子宫指数、MDA、MPO、IL-8、IL-6、IL-2、IL-1β、Caspase-1、GSDMD水平、IκBα、TLR4、NF-κBp65蛋白均上升,LgM、LgG、IgA、SOD水平下降;与模型组相比,中药对照组、益气化瘀方组IL-8、IL-6、IL-2、IL-1β水平及病理综合评分、子宫指数、MPO、MDA、Caspase-1、GSDMD水平、IκBα、TLR4、NF-κBp65蛋白均降低,LgM、LgG、IgA、SOD提升;而与益气化瘀方组相比,益气化瘀方+TAK242组病理综合评分、子宫指数及IL-8、IL-6、IL-2、IL-1β、caspase-1、GSDMD水平、IκBα、TLR4、NF-κBp65、MDA、MPO蛋白均上升明显,LgM、LgG、IgA、SOD水平降低显著,差异有统计学意义(P<0.05)。结论: 益气化瘀方可通过抑制TLR4/NF-κB通路减轻细胞焦亡,调节炎性因子活性,改善氧化应激反应及免疫功能,发挥治疗效果。

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	卢利霞
	林晓华
	杨丹丹

关键词 ：益气化瘀方, Toll样受体4/核因子κB通路, 细胞焦亡, 子宫内膜炎

Abstract：Objective: To explore the regulation of the Toll-like receptor 4 / nuclear factor κB (TLR 4 / NF- κ B) pathway-mediated cell pyroptosis to improve endometritis in rats. Methods: Fifty-five SPF-grade rats were selected as the experimental subjects and divided into five groups of 11 rats each by random number table method, which were grouped into 11 blank groups, model group, traditional Chinese medicine control group, Yiqi Huayuquan group, and Yiqi Huayuquan+TAK242 group, each with 10 rats, after being processed by endometritis modeling. The levels of inflammatory factors, oxidative stress, pathological scores and relative expressions of TLR4 and NF-κB were compared among the rats in each group. Results: Compared with the blank group, the model group, the Chinese medicine control group, and the Yiqi Huayuquan+TAK242 group showed increases in pathological composite scores, uterine index, MDA, MPO, IL-8, IL-6, IL-2, IL-1β, Caspase-1, GSDMD levels, IκBα, TLR4, and NF-κBp65 proteins, and decreases in LgM, LgG, IgA, and SOD levels. Compared with the model group, IL-8, IL-6, IL-2, IL-1β levels and pathological composite scores, uterine index, MPO, MDA, Caspase-1, GSDMD levels, IκBα, TLR4, NF-κBp65 protein were decreased, and LgM, LgG, IgA, SOD were elevated in the traditional Chinese medicine control group and Yiqi Huayuquan group. Compared with the group of Yiqi Huayu Fang + TAK242, the pathology composite score, uterine index and the levels of IL-8, IL-6, IL-2, IL-1β, caspase-1, GSDMD, IκBα, TLR4, NF-κBp65, MDA and MPO proteins were increased significantly, and the levels of LgM, LgG, IgA and SOD were decreased significantly, with a statistically significant difference (P<0.05). Conclusion: Yiqi Huayu Fang can exert therapeutic effects by inhibiting the TLR4/NF-κB pathway to attenuate cellular pyroptosis, regulating the activity of inflammatory factors, and improving oxidative stress and immune function.

Key words： Yiqi huayu recipe Toll-like receptor 4/ nuclear factor κB pathway Pyroptosis Endometritis

基金资助:河北省中医药管理局课题,(编号:2023027)

通讯作者: 杨丹丹

引用本文:

卢利霞, 林晓华, 杨丹丹. 益气化瘀方调节TLR4/NF-κB通路介导细胞焦亡改善大鼠子宫内膜炎[J]. 河北医学, 2025, 31(12): 1969-1676.
LU Lixia, LIN Xiaohua, YANG Dandan. Regulation of TLR4/NF-κB Pathway by Yiqi Huayu Formula Ameliorates Endometritis in Rats by Mediating Cellular Focal Death. HeBei Med, 2025, 31(12): 1969-1676.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.12.06 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I12/1969

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