Abstract：Objective: To study the impacts of long non-coding RNA small nucleolar RNA host gene 1 (LncRNA SNHG1) on the migration,invasion and chemotherapy sensitivity of gastric cancer cells by regulating miR-330-5p/high mobility group A2 (HMGA2) axis. Methods: MGC-803 cells were cultured in vitro and randomly grouped into control group,si-SNHG1 group,pc-NC+miR-330-5p-NC group,and si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; cell scratch and Transwell invasion test were performed to detect cell migration and invasion in each group.MGC-803/oxaliplatin (OXA) cells were cultured in vitro and randomly grouped into control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and transfection,real-time fluorescence quantitative PCR and immunoblotting were performed to detect the expression of LncRNA SNHG1,miR-330-5p,and HMGA2 in each group; CCK-8,Edu staining,and TUNEL staining experiments were conducted to detect cell proliferation and apoptosis in each group.The MGC-803/OXA transplanted tumor nude mouse model was constructed and randomly grouped into a control group,OXA group,OXA+si-SNHG1 group,OXA+pc-NC+miR-330-5p-NC group,and OXA+si-SNHG1+miR-330-5p inhibitor group.After grouping and processing,the tumor volume and weight were measured.Targeted validation was performed using dual luciferase reporter gene assay. Results: Compared with the control group,the expression of LncRNA SNHG1,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p increased (P<0.05).Compared with the si-SNHG1 group,the expression of HMGA2,migration rate,and invasion number in the si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p decreased (P<0.05).Compared with the control group and OXA group,the expression of LncRNA SNHG1,the expression of HMGA2,cell viability,proliferation rate,tumor volume and weight in the OXA+si-SNHG1 group decreased (P<0.05),the expression of miR-330-5p and apoptosis rate increased (P<0.05); there was no obvious change in all indicators of OXA group cells compared to the control group (P>0.05).Compared with the OXA+si-SNHG1 group,the expression of HMGA2,cell viability,proliferation rate,tumor volume,and weight in the OXA+si-SNHG1+miR-330-5p inhibitor group increased (P<0.05),the expression of miR-330-5p and apoptosis rate decreased (P<0.05).LncRNA SNHG1 was able to target the downregulation of miR-330-5p expression in MGC-803 and MGC-803/OXA cells,and miR-330-5p was able to target the downregulation of their HMGA2 expression. Conclusion: Silencing LncRNA SNHG1 can attenuate the expression of HMGA2 by up-regulating miR-330-5p,thereby inhibiting the migration and invasion of gastric cancer cells,enhancing their chemotherapy sensitivity,and enhancing the killing effect of OXA on them.
何毅明, 金爱莲, 周谊佩, 胡永波. LncRNA SNHG1调节miR-330-5p/HMGA2轴对胃癌细胞迁移侵袭和化疗敏感性的影响[J]. 河北医学, 2025, 31(4): 529-537.
HE Yiming, JIN Ailian, ZHOU Yipei, et al. Impacts of LncRNA SNHG1 on Migration Invasion and Chemotherapy Sensitivity of Gastric Cancer Cells by Regulating the miR-330-5p/HMGA2 Axis. HeBei Med, 2025, 31(4): 529-537.
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2025, Vol. 31 
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