病原体靶向测序技术在持续性炎性反应-免疫抑制-分解代谢综合征老年患者中的诊疗价值

doi:10.3969/j.issn.1006-6233.2025.05.07

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摘要 目的: 基于病原体靶向测序(tNGS)技术检测持续性炎性反应-免疫抑制-分解代谢综合征(PICS)老年患者的病原体,评估tNGS筛查该疾病的诊疗价值。方法: 收集2023年7月至2024年7月本院收治的107例一般炎症老年患者以及107例PICS老年患者的痰液样本,采用tNGS检测两组患者病原体,比较两组的微生物特征。结果: 利用tNGS技术,在对照组中检出45种病原体,包括20种细菌、14种病毒以及11种真菌;而观察组中检出48种病原体,包括24种细菌、12种病毒以及12种真菌。采用tNGS技术分离出的病原体中,对照组中细菌检出率居前3位的为具核梭杆菌(n=43)、咽峡炎链球菌群(n=40)和流感嗜血杆菌(n=34);而观察组中则为具核梭杆菌(n=44)和铜绿假单胞菌(n=44)和鲍曼不动杆菌(n=32)。两组中真菌和病毒检出率最高的均是白色念珠菌和EB病毒。结论: tNGS是检测PICS老年患者病原体的潜在工具,能够用于检测PICS患者的病原体并初步确认PICS的临床诊断候选标志物。

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关键词 ：病原体靶向测序技术, 持续性炎性反应-免疫抑制-分解代谢综合征, 肺炎

Abstract：Objective: To detect pathogens in elderly patients with persistent inflammation immunosuppression catabolism syndrome (PICS) using targeted next-generation sequencing (tNGS) technology, and to evaluate the diagnostic and therapeutic value of tNGS in screening for this disease. Methods: A total of 107 sputum samples from general inflammatory elderly patients and 107 sputum samples from PICS elderly patients admitted to our hospital from Jul. 2023 to Jul. 2024 were collected. tNGS was used to detect pathogens in both groups of patients and to compare the microbial characteristics between the two groups. Results: Using tNGS technology, 45 types of pathogens were identified in the control group, including 20 bacteria, 14 viruses, and 11 fungi; while 48 types of pathogens were identified in the observation group, including 24 bacteria, 12 viruses, and 12 fungi. Among the pathogens isolated using tNGS technology, the top three bacteria detected in the control group were Fusobacterium nucleatum (n=43), Streptococcus anginosus group (n=40), and Haemophilus influenzae (n=34); while in the observation group, they were Fusobacterium nucleatum (n=44), Pseudomonas aeruginosa (n=44), and Acinetobacter baumannii (n=32). The most detected fungi and viruses in both groups were Candida albicans and Epstein-Barr virus, respectively. Conclusion: tNGS is a potential tool for detecting pathogens in elderly patients with PICS, which can be used to detect pathogens in the patients and preliminarily confirm clinical diagnostic candidate markers for PICS.

Key words： Targeted next-generation sequencing technelogy Persistent inflammation immunosuppression catabolism syndrome Pneumonia

基金资助:安徽省高校健康智库项目,(编号:2023szk001)

通讯作者: 倪刚

引用本文:

王羊洋, 倪刚, 金吴娟, 余菲, 裴翀. 病原体靶向测序技术在持续性炎性反应-免疫抑制-分解代谢综合征老年患者中的诊疗价值[J]. 河北医学, 2025, 31(5): 743-748.
WANG Yangyang, NI Gang, JIN Wujuan, et al. The Diagnostic and Therapeutic Value of Targeted Next-generation Sequencing Technology in Elderly Patients with Persistent Inflammation Immunosuppression Catabolism Syndrome. HeBei Med, 2025, 31(5): 743-748.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.05.07 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I5/743

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