汉黄芩素调节Wnt/β-catenin信号通路对慢性肾脏病大鼠血管钙化的影响

doi:10.3969/j.issn.1006-6233.2025.08.05

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摘要目的:探讨汉黄芩素(WOG)调节Wnt/β-catenin信号通路对慢性肾脏病(CKD)大鼠血管钙化的影响。方法:随机选择9只大鼠为对照组,其余均建立CKD模型,成功后分为CKD组、WOG+CKD组、WOG+CKD+激活剂组,每组9只。给药8周后,检测各组大鼠的血清中生化指标尿素氮(BUN)、肌酐(SCr)、血清钙(Ca)、磷(P)、碱性磷酸酶(ALP)和全段甲状旁腺激素(iPTH);Von Kossa染色检测主动脉钙化;免疫组化染色检测主动脉组织中Runt相关转录因子2(Runx2);RT-qPCR检测大鼠主动脉组织中Wnt1和β-catenin的mRNA表达;Western blot检测大鼠主动脉组织中Wnt1和β-catenin蛋白表达。结果:与对照组相比,CKD组大鼠血清BUN、SCr、Ca、P、ALP和iPTH水平及主动脉组织中Runx2表达均升高,主动脉严重钙化,Wnt1、β-catenin mRNA和蛋白表达升高(P<0.05);与CKD组相比,WOG+CKD组大鼠血清BUN、SCr、Ca、P、ALP和iPTH水平及主动脉组织中Runx2表达均降低,主动脉钙化减轻,Wnt1、β-catenin mRNA和蛋白表达降低(P<0.05);与WOG+CKD组相比,WOG+CKD+激活剂组大鼠血清BUN、SCr、Ca、P、ALP和iPTH水平及主动脉组织中Runx2表达均升高,主动脉钙化加重,Wnt1、β-catenin mRNA和蛋白表达升高(P<0.05)。结论:WOG可能通过抑制Wnt/β-catenin信号通路减轻CKD大鼠血管钙化。

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关键词 ：汉黄芩素, 慢性肾脏病大鼠, 血管钙化, Wnt/β-catenin信号通路

Abstract：Objective: To investigate the impact of wogonin (WOG) on vascular calcification in chronic kidney disease (CKD) rats by regulating the Wnt/β-catenin signaling pathway. Methods: Nine rats were stochastically selected as control group, while the rest were constructed as CKD models. After successful treatment, they were assigned into CKD group, WOG+CKD group, and WOG+CKD+activator group, with nine rats in each group. After 8 weeks of administration, biochemical indicators such as blood urea nitrogen (BUN), creatinine (SCr), serum calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), and whole parathyroid hormone (iPTH) were detected in the serum of rats in each group. Von Kossa staining was performed to detect aortic calcification. Immunohistochemical staining was performed to detect Runt related transcription factor 2 (Runx2) in aortic tissue. RT-qPCR was performed to detect the mRNA expression of Wnt1 and β-catenin in rat aortic tissue. In addition, Western blot was used to detect the expression of Wnt1 and β-catenin proteins in rat aortic tissue. Results: Compared with the control group, CKD group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, severe aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the CKD group, the WOG+CKD group showed decreased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, reduced aortic calcification, and decreased Wnt1 and β-catenin mRNA and protein expression (P<0.05). Compared with the WOG+CKD group, the WOG+CKD+activator group showed increased BUN, SCr, Ca, P, ALP, and iPTH in serum, Runx2 expression in aortic tissue, worsened aortic calcification, and elevated Wnt1 and β-catenin mRNA and protein expression (P<0.05). Conclusion: WOG may alleviate vascular calcification in CKD rats by inhibiting the Wnt/β-catenin signaling pathway.

Key words： Wogonin Chronic kidney disease rats Vascular calcification Wnt/β-catenin signaling pathway

基金资助:河北省卫生健康委医学科学研究课题计划,(编号:20250263)

通讯作者: 孙福云

引用本文:

刘烨, 孙福云, 田怡, 邢晋, 马晓迎, 生玉平. 汉黄芩素调节Wnt/β-catenin信号通路对慢性肾脏病大鼠血管钙化的影响[J]. 河北医学, 2025, 31(8): 1258-1263.
LIU Ye, SUN Fuyun, TIAN Yi, et al. Impact of Wogonin on Vascular Calcification in Chronic Kidney Disease Rats by Regulating Wnt/β-catenin Signaling Pathway. HeBei Med, 2025, 31(8): 1258-1263.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.08.05 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I8/1258

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