LncRNA SNHG7靶向调控miR-146b-5p对前列腺癌细胞迁移及侵袭的影响

doi:10.3969/j.issn.1006-6233.2025.09.08

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摘要 目的: 探讨长链非编码RNA(LncRNA)核仁小RNA宿主基因7(SNHG7)靶向调控微小RNA-146b-5p(miR-146b-5p)对前列腺癌(PCa)细胞迁移及侵袭的影响。方法: qRT-PCR法测定2023年8月至2024年4月在我院行PCa手术的42例PCa患者癌组织及癌旁组织中LncRNA SNHG7、miR-146b-5p的表达;以对数期DU145细胞为研究对象,将其分为正常组、NC-shRNA组、SNHG7-shRNA组、SNHG7-shRNA+anti-NC组、SNHG7-shRNA+anti-miR-146b-5p组。MTT检测、划痕实验、Transwell实验分别检测DU145细胞增殖、迁移、侵袭;Western blot检测蛋白表达。结果: PCa组织LncRNA SNHG7表达高于癌旁组织(t=30.622,P<0.001),miR-146b-5p表达低于癌旁组织(t=26.869,P<0.001)。SNHG7-shRNA组A490值、划痕愈合率、侵袭数、LncRNA SNHG7、Vimentin表达低于NC-shRNA组、Control组,miR-146b-5p、E-cadherin表达高于NC-shRNA组、Control组(P<0.05);与SNHG7-shRNA组、SNHG7-shRNA+anti-NC组相比,SNHG7-shRNA+anti-miR-146b-5p组A490值、划痕愈合率、侵袭数、LncRNA SNHG7、Vimentin表达升高,miR-146b-5p、E-cadherin表达降低(P<0.05)。LncRNA SNHG7可以靶向负调控miR-146b-5p。结论: 沉默LncRNA SNHG7可能靶向负调控miR-146b-5p抑制PCa细胞的迁移和侵袭。

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关键词 ：长链非编码, 核仁小RNA宿主基因7, 微小RNA-146b-5p, 前列腺癌, 迁移, 侵袭

Abstract：Objective: To investigate the effects of long non-coding RNA (LncRNA) small nucleolar RNA host gene 7 (SNHG7) on migration and invasion of prostate cancer (PCa) cells through targeted regulation of microRNA-146b-5p (miR-146b-5p). Methods: QRT-PCR was used to measure the expression of LncRNA SNHG7 and miR-146b-5p in cancer tissues and adjacent tissues of 42 PCa patients who underwent PCa surgery in our hospital from August 2023 to April 2024. The logarithmic phase DU145 cells were grouped into normal group, NC-shRNA group, SNHG7-shRNA group, SNHG7-shRNA+anti-NC group, and SNHG7-shRNA+anti-miR-146b-5p group. MTT assay, scratch assay, and Transwell assay were used to detect the proliferation, migration, and invasion of DU145 cells, respectively. In addition, Western blot was used to detect the expression of epithelial mesenchymal transition (E-cadherin, Vimentin) proteins in DU145 cells. Results: The expression of LncRNA SNHG7 in PCa tissue was higher than that in adjacent tissues (t=30.622, P<0.001), and the expression of miR-146b-5p was lower than that in adjacent tissues (t=26.869, P<0.001). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA group were lower than those in NC-shRNA group and Control group, while miR-146b-5p and E-cadherin were higher than those in NC-shRNA group and Control group (P<0.05). The A490 value, scratch healing rate, invasion number, LncRNA SNHG7, and Vimentin expression in SNHG7-shRNA+anti-miR-146b-5p group were higher than those in SNHG7-shRNA group and SNHG7-shRNA+anti-NC group, while miR-146b-5p and E-cadherin were lower (P<0.05). LncRNA SNHG7 could target negative regulation of miR-146b-5p. Conclusion: Silencing LncRNA SNHG7 can inhibit the migration and invasion of PCa cells, and it may be achieved by targeting negative regulation of miR-146b-5p.

Key words： Long non-coding Small nucleolar RNA host gene 7 MicroRNA-146b-5p Prostate cancer Migration Invasion

基金资助:河北省卫生健康委医学科学研究,(编号:20221853)

引用本文:

田莉, 崔海军, 徐晋珩, 胡月明, 赵济华, 曹渤海. LncRNA SNHG7靶向调控miR-146b-5p对前列腺癌细胞迁移及侵袭的影响[J]. 河北医学, 2025, 31(9): 1452-1458.
TIAN Li, et al. Effects of LncRNA SNHG7 on Migration and Invasion of Prostate Cancer Cells through Targeted Regulation of miR-146b-5p. HeBei Med, 2025, 31(9): 1452-1458.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.09.08 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I9/1452

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