Abstract：Objective: To explore the effects of alpinetin (ALP) on the proliferation and invasion of prostate cancer (PC) cells by regulating LncRNA FTX/miR-335-5p axis. Methods: The human prostate cancer cell line LNCaP was cultured in vitro, and the MTT colorimetric method was used to detect the proliferative activity of ALP on LNCaP cells. LNCaP cells were assigned into NC group, ALP group, ALP+pcDNA3.1-NC group, and ALP+pcDNA3.1-FTX group. CCK-8 and clone formation experiments were used to detect the proliferation ability of LNCaP cells in each group. Transwell method was used to detect the invasion ability of LNCaP cells in each group. qRT-PCR was used to detect LncRNA FTX and miR-335-5p of LNCaP cells in various groups. Western blot experiment was performed to detect cell proliferation and invasion related proteins. Dual luciferase reporter gene assay was performed to detect the targeting relationship between LncRNA FTX and miR-335-5p. Construct a nude mouse PC transplant tumor model, detect the mass and volume of tumors in each group, and measure the expression levels of LncRNA FTX and miR-335-5p in tumor tissues of each group. Results: For the NC group, the ALP group and ALP+pcDNA3.1-NC group had conspicuously lower LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously higher miR-335-5p (P<0.05). For the ALP+pcDNA3.1-NC group, the ALP+pcDNA3.1-FTX group had conspicuously higher LNCaP cell survival rate, clone formation number, cell invasion number, LncRNA FTX, cyclin D1 (CCND1), myelocytomatosis viral oncogene homolog (MYC), matrix metalloproteinase-2 (MMP) -2 and MMP-9 mRNAs and proteins (P<0.05), and conspicuously lower miR-335-5p (P<0.05). The dual luciferase reporter gene experiment showed that LncRNA FTX targeted negative regulation of miR-335-5p (P<0.05). The results of the nude mouse tumorigenesis experiment showed that compared with the Model group, the ALP group had significantly smaller tumor mass and volume, and the expression of LncRNA FTX in tumor tissue was significantly downregulated, while the expression of miR-335-5p was significantly up-regulated (P<0.05). Conclusion: ALP may inhibit proliferation and invasion of PC cells by regulating LncRNA FTX/miR-335-5p axis.
雷晓, 毕根旺, 贾兰, 马凰斌. 山姜素调节LncRNA FTX/miR-335-5p轴对前列腺癌细胞增殖及侵袭的影响[J]. 河北医学, 2025, 31(9): 1458-1465.
LEI Xiao, BI Genwang, JIA Lan, et al. Effects of Alpinetin on the Proliferation and Invasion of Prostate Cancer Cells by Regulating LncRNA FTX/miR-335-5p Axis. HeBei Med, 2025, 31(9): 1458-1465.
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2025, Vol. 31 
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