Abstract：Objective: To analyze the relationship between the expression levels of microRNA 7 (miR-7), miR-21 and the proliferation, metastasis and angiogenesis of renal clear cell carcinoma (ccRCC). Methods: Collect ccRCC tissue and para-cancer tissue, culture ccRCC cell lines 786-O, RCC23, ACHN, SN12-PM6, A498 and human renal proximal tubule cell line HK-2 in vitro, and detect the expression level of miR-7 and miR-21 by qRT-PCR. A498 cell line was selected for miR-7 overexpression treatment (miR-7-mimics NC group, miR-7-mimics group), and 786-O cell line was selected for miR-21 knockdown treatment (miR-21-inhibitorsNC group, miR-21-inhibitorsgroup). The expression levels of epithelial-mesenchymal transition-related proteins were detected by Western blot, cell proliferation activity was detected by EDU proliferation assay, cell migration activity was detected by scratch assay, and cell invasion activity was detected by Transwell assay. The supernatants of the above groups of cells were co-cultured with human umbilical vein endothelial cells (HUVEC) respectively (miR-7-mimicsNC supernatant group, miR-7-mimics supernatant group, miR-21-inhibitorsNC supernatant group, miR-21-inhibitors supernatant group), and the differences in the angiogenic ability of HUVEC cells in each group were observed. Results: Compared with para-cancerous tissue, miR-7 levels were decreased and miR-21 levels were increased in ccRCC cancer tissue. Compared with HK-2 cells, miR-7 levels were decreased and miR-21 levels were increased in ccRCC cell lines. Compared with the miR-7-mimicsNC group, the protein expression levels of Vimentin and N-cadherin in the miR-7-mimics group were decreased, and the protein levels of E-cadherin and ZO-1 were increased, and the cell proliferation, migration and invasion activities were all decreased. Compared with the miR-21-inhibitorsNC group, the protein expression levels of Vimentin and N-cadherin in the miR-21-inhibitors group were decreased, the protein levels of E-cadherin and ZO-1 were increased, and the cell proliferation, migration and invasion activities were all decreased. Compared with the miR-7-mimicsNC supernatant group, the miR-7-mimics supernatant group had weaker angiogenic ability of HUVEC cells; compared with the miR-21-inhibitorsNC supernatant group, the miR-21-inhibitors supernatant group had weaker angiogenic ability of HUVEC cells. Conclusion: miR-7 was down-regulated and miR-21 was up-regulated in ccRCC. Overexpression of miR-7 or knockdown of miR-21 could inhibit the proliferation, metastasis and angiogenesis of ccRCC cells.
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2025, Vol. 31 
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