Abstract：Objective: To explore the effects of ligustilide on hypoxia/reoxygenation induced myocardial cell injury by adjusting Notch1/Hes1 signaling pathway. Methods: H9C2 cardiomyocytes were used as the research object and grouped: cells with normal culture were labeled as the normal group, hypoxia/reoxygenation induction as the hypoxia/reoxygenation group, hypoxia/reoxygenation induction+10μmoL/L ligustilide as the hypoxia/reoxygenation+low-dose ligustilide group, hypoxia/reoxygenation induction+20μmoL/L ligustilide as the hypoxia/reoxygenation+medium-dose ligustilide group, hypoxia/reoxygenation induction+40μmoL/L ligustilide as the hypoxia/reoxygenation+high-dose ligustilide group, and hypoxia/reoxygenation induction+40μmoL/L ligustilide+5 mg/L Notch activator Jagged1 as the hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group. MTT, flow cytometry, ELISA, and Western blot were used to detect cell proliferation, apoptosis, LDH, CK, AST, IL-18, IL-1β levels, and the Notch1/Hes1 signaling pathway related proteins. Results: The hypoxia/reoxygenation group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the normal group (P<0.05). The hypoxia/reoxygenation+low, medium, and high-dose ligustilide groups showed increased OD value, and decreased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation group (P<0.05). The hypoxia/reoxygenation+high-dose ligustilide+Jagged1 group showed decreased OD value, and increased cell apoptosis rate, LDH, CK, AST, IL-18, IL-1β levels, Notch1, and Hes1 proteins than the hypoxia/reoxygenation+high-dose ligustilide group. Conclusion: The improvement effect of ligustilide on hypoxia/reoxygenation induced myocardial cell injury may be achieved by inhibiting Notch1/Hes1 signaling pathway.
[1] 刘孟楠,毛琳慎,李子一,等.丹参饮通过调节线粒体mPTP开放改善缺氧/复氧心肌细胞损伤的机制研究[J].中药材,2024,47(12):3108-3113. [2] 赵玲琳,张勇,薛慧,等.麦冬皂苷D对大鼠心肌细胞缺氧复氧损伤的保护作用及机制研究[J].中国循环杂志,2022,37(2):178-184. [3] 赵香梅,徐雅欣,陈龙,等.藁本内酯通过调控miR-133b-5p表达对脂多糖所致心肌损伤的保护作用[J].中国医院药学杂志,2020,40(18):5-9. [4] Duan H,Shen F,Li L et al.Activation of the Notch signaling pathway in the anterior cingulate cortex is involved in the pathological process of neuropathic pain[J].Pain,2021,162(1):263-274. [5] 刘婷婷,董红艳,马艳兰,等.D-β-羟基丁酸通过激活Notch1/Hes1通路和抑制内质网应激改善大鼠急性心肌梗死[J].西部医学,2022,34(12):1736-1742. [6] Yuan Y,Huang H,Hu T,et al.Curcumin pretreatment attenuates myocardial ischemia/reperfusion injury by inhibiting ferroptosis,autophagy and apoptosis via HES1[J].Int Mol Med,2024,54(6):110-123. [7] 秦少强,梁惠清,王晓元,等.miR-202-5p靶向下调SOX6表达对缺氧复氧诱导的心肌细胞氧化应激的影响研究[J].中国免疫学杂志,2021,37(5):541-546. [8] 张岚,武永新,张涛,等.藁本内酯调控PKD1/HIF-1α/VEGF通路对心力衰竭大鼠的改善作用[J].吉林大学学报(医学版),2024,50(1):42-49. [9] Ren C H,Li N,Gao C,et al.Ligustilide provides neuroprotection by promoting angiogenesis after cerebral ischemia[J].Neurol Res,2020,42(8):483-692. [10] Zhou Z,Liu Z,Zhang C,et al.Mild hypothermia alleviates early brain injury after subarachnoid hemorrhage via suppressing pyroptosis through AMPK/NLRP3 inflammasome pathway in rats[J].Brain Res Bull,2023,193(1):72-83. [11] Lin Y W,Li X X,Fu F H,et al.Notch1/Hes1PTEN/AKT/IL17A feedback loop regulates Th17 cell differentiation in mouse psoriasislike skin inflammation[J].Mol Med Rep,2022,26(1):223-235. [12] Wang L,Lai S,Zou H,et al.Ischemic preconditioning/ischemic postconditioning alleviates anoxia/reoxygenation injury via the Notch1/Hes1/VDAC1 axis[J].Biochem Mol Toxicol,2022,36(11):e23199-e23206. [13] Lu Y,Li S Y,Lou H.Patchouli alcohol protects against myocardial ischaemia-reperfusion injury by regulating the Notch1/Hes1 pathway[J].Pharm Biol,2022,60(1):949-957.
2025, Vol. 31 
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