miR-182-3p miR-195-5p miR-200a-3p在妊娠期高血压疾病患者血清中的表达及对早产的预测价值

doi:10.3969/j.issn.1006-6233.2025.11.015

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摘要 目的: 探讨微小RNA(miR)-182-3p、miR-195-5p、miR-200a-3p在妊娠期高血压疾病(HDP)患者血清中的表达及对早产的预测价值。方法: 选取2022年6月至2024年6月在本院妇产科诊治的391例HDP患者为研究组,根据疾病严重程度分为单纯妊娠期高血压165例,轻度子痫前期134例,重度子痫前期92例;根据患者早产发生情况分为早产组107例(28周≤妊娠时间<37周),非早产组284例(37周≤妊娠时间<42周),同期选取316例正常足月生产的健康孕妇作为对照组。实时荧光定量PCR法检测血清miR-182-3p、miR-195-5p、miR-200a-3p水平;多因素Logistic回归分析影响HDP患者发生早产的因素;受试者工作特征曲线(ROC)分析血清miR-182-3p、miR-195-5p、miR-200a-3p对HDP患者早产的预测价值。结果: 与对照组相比,研究组血清miR-182-3p、miR-200a-3p水平升高,miR-195-5p水平降低(P<0.05);重度子痫前期HDP患者血清miR-182-3pp、miR-200a-3p水平显著高于轻度子痫前期和单纯妊娠期高血压(P<0.05),miR-195-5水平显著低于轻度子痫前期和单纯妊娠期高血压(P<0.05);早产组收缩压、舒张压、重度子痫前期占比、24h UPQ、Scr以及血清miR-182-3p、miR-200a-3p水平均显著高于非早产组(P<0.05),血清miR-195-5p水平显著低于非早产组(P<0.05);疾病严重程度、24h UPQ以及血清miR-182-3p、miR-195-5p、miR-200a-3p是影响HDP患者发生早产的因素(P<0.05);血清miR-182-3p、miR-195-5p、miR-200a-3p三者联合预测HDP患者早产的AUC为0.903,三者联合优于各自单独预测,P<0.05)。结论: HDP患者血清miR-182-3p、miR-200a-3p水平升高,miR-195-5p水平降低,三者联合对预测HDP患者合并早产具有一定的临床应用价值。

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	甄斐斐
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关键词 ：微小RNA-182-3p, 微小RNA-195-5p, 微小RNA-200a-3p, 妊娠期高血压, 早产

Abstract：Objective: To investigate the expression of microRNAs (miRNAs)-182-3p, miR-195-5p, and miR-200a-3p in the serum of patients with hypertensive disorders of pregnancy (HDP) and their predictive value for premature birth. Methods: A total of 391 patients with HDP treated in the obstetrics and gynecology department of our hospital from June 2022 to June 2024 were regarded as study group. Complying with severity of the disease, they were classified into 165 cases of simple HDP, 134 cases of mild preeclampsia, and 92 cases of severe preeclampsia. Complying with occurrence of premature birth in patients, they were grouped into a premature birth group of 107 cases (28 weeks ≤ gestational time<37 weeks) and a non premature birth group of 284 cases (37 weeks ≤ gestational time<42 weeks). During the same period, 316 healthy pregnant women who gave birth at full term were made the control group. Real-time fluorescence quantitative PCR was used to detect serum miR-182-3p, miR-195-5p, and miR-200a-3p. Multivariate logistic regression was used to analyze the factors influencing premature birth in patients with HDP. Receiver operating characteristic curves (ROC) were used to analyze the predictive value of serum miR-182-3p, miR-195-5p, and miR-200a-3p for premature birth in HDP patients. Results: Compared with control group, study group had higher serum miR-182-3p and miR-200a-3p, and lower miR-195-5p (P<0.05). Serum miR-182-3pp and miR-200a-3p in patients with severe preeclampsia were higher than those in patients with mild preeclampsia and simple HDP (P<0.05), while miR-195-5 was lower (P<0.05). The systolic blood pressure, diastolic blood pressure, proportion of severe preeclampsia, 24-hour UPQ, Scr, serum miR-182-3p, and miR-200a-3p in premature birth group were higher than those in non premature birth group (P<0.05), while serum miR-195-5p was lower than that in non premature birth group (P<0.05). The severity of the disease, 24h UPQ, serum miR-182-3p, miR-195-5p, and miR-200a-3p were factors that affected premature birth in HDP patients (P<0.05). The AUC of the joint of serum miR-182-3p, miR-195-5p, and miR-200a-3p in predicting premature birth in HDP patients was 0.903, and the joint of the three was better than their individual predictions P<0.05). Conclusion: The levels of miR-182-3p and miR-200a-3p in serum of HDP patients are elevated, while the level of miR-195-5p is reduced. The joint of the three has certain clinical application value in predicting premature birth in HDP patients.

Key words： MicroRNA-182-3p MicroRNA-195-5p MicroRNA-200a-3p Hypertensive disorders of pregnancy Premature birth

基金资助:山西省卫生健康科研课题项目,(编号:2023A072)

通讯作者: 魏涛

引用本文:

甄斐斐, 闫翠云, 魏涛. miR-182-3p miR-195-5p miR-200a-3p在妊娠期高血压疾病患者血清中的表达及对早产的预测价值[J]. 河北医学, 2025, 31(11): 1849-1854.
ZHEN Feifei, YAN Cuiyun, WEI Tao. The Expression of miR-182-3p miR-195-5p miR-200a-3p in the Serum of Patients with Hypertensive Disorders of Pregnancy and the Predictive Value for Premature Birth. HeBei Med, 2025, 31(11): 1849-1854.

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http://www.hbyxzzs.cn/CN/10.3969/j.issn.1006-6233.2025.11.015 或 http://www.hbyxzzs.cn/CN/Y2025/V31/I11/1849

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