Abstract：Objective: To investigate the protective effect of oridonin on type 2 diabetic osteoporosis (DOP) in rats by modulating Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway. Methods: Nine rats were randomly labeled as control group from 36 rats, while the rest were induced to establish rat DOP models through a high-glucose and high-fat diet combined with STZ induction before they were assigned into DOP group, Oridonin group, and Oridonin+activator (Coumermycin A1) group, with 9 rats in each group and 10-week drug administration. After administration, enzyme-linked immunosorbent assay (ELISA) was used to detect serum bone turnover markers osteocalcin (OCN) and procollagen type I amino-terminal propeptide (PINP). Microscopic computed tomography was performed to detect femur related parameters. Biomechanics was performed to test the maximum load of the femur. HE staining was used to observe femoral tissue. RT-qPCR was used to detect the expression of JAK2 and STAT3 mRNA in femurs. Western blot was performed to detect the expression of JAK2 and STAT3 proteins in femurs. Results: Compared with control group, the bone turnover markers PINP and OCN reduced in DOP group, the bone mineral density (BMD), number of bone trabeculae (Tb.N), thickness of bone trabeculae (Tb.Th), bone volume/tissue volume (BV/TV), and maximum load of femur reduced, the JAK2, STAT3 mRNA and protein expression increased (P<0.05), and the femur structure was disordered, with thinning and reduced number of bone trabeculae. Compared with DOP group, the PINP and OCN increased in Oridonin group, the femoral BMD, Tb.N. Tb.Th, BV/TV, and maximum load increased, the JAK2, STAT3 mRNA and protein expression decreased (P<0.05), and the symptoms of osteoporosis were alleviated. In addition, JAK2 activator reversed the effect of Oridonin on DOP rats (P<0.05). Conclusion: Oridonin may have a protective effect on DOP rats by inhibiting JAK2/STAT3 signaling pathway.
杨浩宇, 朱广浩, 饶志远, 白艳甫. 冬凌草甲素调节JAK2/STAT3信号通路对2型糖尿病骨质疏松大鼠的保护作用[J]. 河北医学, 2026, 32(2): 177-182.
YANG Haoyu, ZHU Guanghao, RAO Zhiyuan, et al. Protective Effects of Oridonin on Type 2 Diabetic Osteoporotic Rats via Modulation of the JAK2/STAT3 Signalling Pathway. HeBei Med, 2026, 32(2): 177-182.
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2026, Vol. 32 
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