|
|
|
| Expression Level of DNMT1 in Lung Adenocarcinoma and Its Correlation with TFF1 Gene Methylation Level and Clinical Significances |
| GUO Chao, et al |
| Baoding No.2 Central Hospital, Hebei Baoding 072750,China |
|
|
|
|
Abstract Objective: To measure the expression level of DNA methyltransferase 1 (DNMT1) and the methylation level of trefoil factor 1 (TFF1) in lung adenocarcinoma,and to analyze their clinical significances. Methods: The methylation level of TFF1 in human lung adenocarcinoma tissues was detected by pyrosequencing.The mRNA levels of TFF1 and DNMT1 were detected by reverse transcription coupled to the quantitative polymerase chain reaction (RT-qPCR).Their protein levels were detected by immunohistochemical staining and Western blot.The correlation of the methylation level of TFF1 and expression level of DNMT1 with pathological characteristics of lung adenocarcinoma was explored. Results: The methylation rate of the TFF1 gene was significantly higher in the lung adenocarcinoma tissues compared with that of paracancerous tissues (P<0.05).PCR results showed that the mRNA level of TFF1 was significantly lower in the lung adenocarcinoma tissues,while that of DNMT1 was significantly higher than those of paracancerous tissues (P<0.05).Immunohistochemistry showed a significantly lower protein level of TFF1,but higher protein level of DNMT1 in the lung adenocarcinoma tissues than those of paracancerous tissues (P<0.05).Western blot results showed a significantly lower protein level of TFF1,but higher protein level of DNMT1 in the lung adenocarcinoma tissues than those of paracancerous tissues (P<0.05).TFF1 methylation level and DNMT1 expression were significantly correlated with the TNM staging and lymph node metastasis of lung adenocarcinoma (P<0.05).The statistical analysis showed that TFF1 methylation and DNMT1 protein expression were related in lung adenocarcinoma tissues, it was positive correlation (r=0.538, P<0.05). Conclusion: During the cancerous progression of lung adenocarcinoma,DNMT1 is closely linked with methylation of the TFF1 gene.High level of DNMT1 and high methylation level of TFF1 both promote the occurrence and development of lung adenocarcinoma.
|
|
|
|
|
|
[1] Wei X,Li X,Hu S,et al.Regulation of ferroptosis in lung adenocarcinoma[J].Int Mol Sci,2023,24(19):14614.
[2] Wu J,Lin Z.Non-small cell lung cancer targeted therapy: drugs and mechanisms of drug resistance[J].Int Mol Sci,2022,23(23):15056.
[3] Shekarriz R,Kochakli N,Eslami-Jouibari M,et al.TFF1 gene single nucleotide polymorphism (rs3761376) and colorectal cancer risk[J].Mol Biol Rep,2022,49(10):10127-10131.
[4] Hoffmann W. Self-renewal and cancers of the gastric epithelium: an update and the role of the lectin TFF1 as an antral tumor suppressor[J]. Int Mol Sci,2022,23(10):5377.
[5] Qian Z,Jiang Y,Shou C,et al.Validation of the DNA methylation landscape of TFF1/TFF2 in gastric cancer[J].Cancers (Basel),2022,14(22):5474.
[6] 李亚光,邓同兴,赵克芳,等.稳定过表达TFF1对MHCC97H细胞增殖、侵袭、转移及EMT发生的影响[J].中国免疫学杂志,2021,37(14):1733-1737.
[7] 杨晨,任长明,寇宗强,等.血清肿瘤坏死因子受体相关蛋白1、三叶因子1表达水平对卵巢癌患者化疗疗效的预测价值[J].齐齐哈尔医学院学报,2024,45(10):934-939.
[8] 王真真,邓青,付云.血清TFF1、SLPI、PGRMC1水平对乳腺癌的诊断价值及与病理因素的相关性分析[J].淮海医药,2023,41(6):571-575.
[9] Busch MA,Haase A,Miroschnikov N,et al.TFF1 in aqueous humor-A potential new biomarker for retinoblastoma[J].Cancers (Basel),2022,14(3):677.
[10] Yusufu A,Shayimu P,Tuerdi R,et al.TFF3 and TFF1 expression levels are elevated in colorectal cancer and promote the malignant behavior of colon cancer by activating the EMT process[J].Int Oncol,2019,55(4):789-804.
[11] 王敏丽,邓同兴,陈彦锋,等.TFF1甲基化状态对食管鳞状细胞癌TE1和TE13细胞凋亡的影响[J].现代预防医学,2021,48(8):1468-1472.
[12] Doi R,Fukumura Y,Lu R,et al.DNMT1 expression and DNA methylation in intraductal papillary neoplasms of the bile duct[J].Anticancer Res,2022,42(6):2893-2902. |
|
|
|
2025, Vol. 31 
