羟基红花黄色素A通过Hippo途径调控糖酵解影响结直肠癌细胞增殖侵袭和凋亡

doi:10.3969/j.issn.1006-6233.2025.07.01

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Abstract Objective: To investigate the effect of hydroxysafflor yellow A(HSYA) on the proliferation invasion and apoptosis of colorectal cancer HT29 cells and its mechanism. Methods: HT29 cells were divided into 5 groups: Con group (HT29 cells), HSYA low dose group (HSYA-L group, HT29+20 μmoL/L HSYA), HSYA medium dose group (HSYA-M group, HT29+ 20 μmoL/L HSYA), HSYA high dose group (HSYA-H group, HT29+20 μmoL/L HSYA), HSYA high dose group (HSYA-H group, HT29+30 μmoL/L HSYA) HT29+40 μmoL/L HSYA) and XMU-MP-1 group (Hippo inhibitor XMU-MP-1, HT29+40 μmoL/L HSYA+3 μmoL/L XMU-MP-1). The effect of HSYA on the proliferative activity of HT29 cells was detected by CCK-8 and the half-inhibitory concentration (IC₅₀) was calculated. The proliferation, migration and invasion ability of HT29 cells were detected by EdU proliferation assay, cell scratch assay and Transwell assay. The apoptosis rate was detected by flow cytometry. Glucose consumption and lactic acid production in HT29 cells were detected by corresponding kits. The expression levels of glycolysis and Hippo pathway related proteins were detected by Western Blot. Results: Compared with Con group, cell viability, proliferation rate, mobility, number of transmembrane cells, glucose consumption, lactic acid production, HXK2 and LDHA in HSYA-L, HSYA-M and HSYA-H groups were significantly decreased (P<0.05). The apoptosis rate, p-MST1/2, p-LATS1/2, YAP and TAZ levels were significantly increased (P<0.05) in a dose-dependent manner. Compared with XMU-MP-1 group, cell viability, proliferation rate, mobility, number of transmembrane cells, glucose consumption, lactic acid production, HXK2 and LDHA in HSYA-H group were significantly decreased (P<0.05), while apoptosis rate, p-MST1/2, p-LATS1/2, YAP and TAZ levels were significantly increased (P<0.05). Conclusion: HSYA can significantly inhibit the proliferation, migration and invasion of HT29 cells and induce cell apoptosis, which may be related to the activation of Hippo pathway and the regulation of glycolysis process.

Key words： Hydroxysafflor yellow A Hippo pathway Glycolysis Colorectal cancer Cell proliferation Cell invasion

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	ZHOU Chu
	ZHOU Yu
	AN Ling
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ZHOU Chu,ZHOU Yu,AN Ling, et al. Hydroxylsafflower Yellow A Affecting the Proliferation Invasion and Apoptosis of Colorectal Cancer Cells by Regulating Glycolysis through Hippo Pathway[J]. HeBei Med, 2025, 31(7): 1057-1063.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.07.01 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I7/1057

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