胃癌组织中miR-1297 BCAT1表达与病理参数上皮-间充质转化及预后的关系

doi:10.3969/j.issn.1006-6233.2025.07.016

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Abstract Objective: To investigate the relationship between the expression of microRNA-1297 (miR-1297) and branched-chain amino acid transaminase 1 (BCAT1) in gastric cancer tissues with pathological parameters, epithelial-mesenchymal transition (EMT) and prognosis. Methods: A total of 150 gastric cancer patients who underwent surgical resection from January 2017 to September 2019 (gastric cancer group) and 65 patients undergoing gastrointestinal polyp surgery during the same period (control group) were selected. The expression of miR-1297, BCAT1 mRNA, and EMT markers [vimentin (VIM), snail family transcriptional repressor 1 (SNAI1), E-cadherin (E-cad), N-cadherin (N-cad)] in gastric cancer and gastrointestinal polyp tissues were detected using real-time quantitative polymerase chain reaction. Binding sites between miR-1297 and BCAT1 were predicted through an online database, and the correlation between the expression of miR-1297, BCAT1 mRNA, and EMT markers in gastric cancer tissues was analyzed using Pearson correlation analysis. Patients were grouped into high and low expression groups based on the mean expression levels of miR-1297 and BCAT1 mRNA, and Kaplan-Meier survival curves were plotted for patients with different miR-1297 and BCAT1 mRNA expression levels. Multivariate Cox regression analysis was used to explore the relationship between miR-1297, BCAT1 mRNA expression, and prognosis in gastric cancer patients. Results: Compared with gastrointestinal polyp tissues, miR-1297 and E-cad mRNA expression levels were lower in gastric cancer tissues, while BCAT1 mRNA, VIM mRNA, SNAI1 mRNA, and N-cad mRNA expression levels were higher (P<0.05). A binding site was identified at the 3' untranslated region (4135-4142) of BCAT1 for miR-1297. In gastric cancer tissues, miR-1297 expression was negatively correlated with BCAT1 mRNA, VIM mRNA, SNAI1 mRNA, and N-cad mRNA expression but positively correlated with E-cad mRNA expression (P<0.05). BCAT1 mRNA expression was positively correlated with VIM mRNA, SNAI1 mRNA, and N-cad mRNA but negatively correlated with E-cad mRNA (P<0.05). miR-1297 expression was lower and BCAT1 mRNA expression higher in poorly differentiated, TNM stage III, and lymph node metastatic gastric cancer tissues compared to moderately and well-differentiated, TNM stage I-II, and non-metastatic tissues (P<0.05). The 3-year overall survival rate of 150 gastric cancer patients was 61.33% (92/150). Patients with high miR-1297 expression had higher 3-year overall survival rates than those with low miR-1297 expression, while patients with high BCAT1 mRNA expression had lower survival rates compared to those with low BCAT1 mRNA expression (P<0.05). Poor differentiation, TNM stage III, lymph node metastasis, and BCAT1 mRNA ≥2.38 were independent risk factors for mortality, while miR-1297 ≥1.33 was an independent protective factor (P<0.05). Conclusion: miR-1297 expression is low and BCAT1 mRNA expression is high in gastric cancer tissues, and both are associated with unfavorable pathological characteristics, EMT, and poor prognosis. These factors may serve as novel biomarkers for evaluating EMT and prognosis in gastric cancer patients.

Key words： Gastric cancer MicroRNA-1297 Branched-chain amino acid transaminase 1 Pathological parameters Epithelial-mesenchymal transition

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	ZHANG Xia
	DENG Shuangnian
	YANG Xiaojuan

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ZHANG Xia,DENG Shuangnian,YANG Xiaojuan. Correlation of miR-1297 and BCAT1 Expression with Pathological Parameters Epithelial-Mesenchymal Transition and Prognosis in Gastric Cancer Tissues[J]. HeBei Med, 2025, 31(7): 1145-1151.

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http://www.hbyxzzs.cn/EN/10.3969/j.issn.1006-6233.2025.07.016 OR http://www.hbyxzzs.cn/EN/Y2025/V31/I7/1145

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